mCherry-Tag(PNF001) Nb-FC recombinant antibody

    • Catalog No.:YA0648
    • Applications:WB
    • Reactivity:Species independent
      • Target:
      • mCherry-Tag
      • Immunogen:
      • Recombinant Protein of mCherry-Tag
      • Specificity:
      • The antibody detects mCherry tag fusion proteins.
      • Formulation:
      • Phosphate-buffered solution
      • Source:
      • Camel, chimeric fusion of Nanobody (VHH) and mouse IgG1 Fc domain , recombinantly produced from 293F cell
      • Dilution:
      • WB 1:1000-5000
      • Purification:
      • Recombinant Expression and Affinity purified
      • Storage Stability:
      • -15°C to -25°C/1 year(Avoid freeze / thaw cycles)
      • Other Name:
      • mCherry;mCherry Tag;
      • Background:
      • mCherry is derived from proteins originally isolated from Cnidarians (jelly fish, sea anemones and corals), and is used as a fluorescent tracer in trasfection and transgenic experiments. The prototype for these fluorescent proteins is Green Fluorescent Protein (GFP), which is a ~27kDa protein isolated originally from the jellyfish Aequoria victoria. The mCherry protein is derived from DsRed, a red fluorescent protein related to GFP isolated from so-called disc corals of the genus Discosoma.
      • Products Images
      • Whole extracts were separated by 10% SDS-PAGE, and the membrane was blotted with anti-mCherry-Tag(PNF001)antibody. The HRP-conjugated Goat anti-Mouse IgG(H + L) antibody was used to detect the antibody. Lane 1: BL21 transfected with an empty expression vector Lane 2: BL21 transfected with a mCherry tagged protein Predicted band size: 38kDa Observed band size: 38kDa