Vimentin Polyclonal Antibody

    • Catalog No.:YT5796
    • Applications:WB,ELISA
    • Reactivity:Human,Mouse,Rat
    • Description
    • Gene Name:
    • VIM
    • Protein Name:
    • Vimentin
    • Human Gene Id:
    • 7431
    • Human Swiss Prot No:
    • P08670
    • Mouse Swiss Prot No:
    • P20152
    • Immunogen:
    • The antiserum was produced against synthesized peptide derived from the C-terminal region of human VIM. AA range:371-420
    • Specificity:
    • Vimentin Polyclonal Antibody detects endogenous levels of Vimentin
    • Formulation:
    • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
    • Source:
    • Rabbit
    • Dilution:
    • WB 1:500-2000, ELISA 1:10000-20000
    • Purification:
    • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
    • Concentration:
    • 1 mg/ml
    • Storage Stability:
    • -20°C/1 year
    • Other Name:
    • Vimentin
    • MolecularWeight(Da):
    • 53652
    • Observed Band(KD):
    • 55
    • Background:
    • VIM (vimentin) encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by VIM is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in VIM causes a dominant, pulverulent cataract.
    • Function:
    • cell motion, intermediate filament-based process,
    • Products Images
    • Western Blot analysis of Hela cells using Vimentin Polyclonal Antibody diluted at 1:500. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
    • Immunohistochemical analysis of paraffin-embedded human-breast-cancer, antibody was diluted at 1:200
    • Immunohistochemical analysis of paraffin-embedded human-breast-cancer, antibody was diluted at 1:200
    • Immunohistochemical analysis of paraffin-embedded human-tonsil, antibody was diluted at 1:200
    • Immunohistochemical analysis of paraffin-embedded human-tonsil, antibody was diluted at 1:200