The antiserum was produced against synthesized peptide derived from human Elk1 around the phosphorylation site of Ser389. AA range:356-405

Phospho-Elk-1 (S389) Polyclonal Antibody detects endogenous levels of Elk-1 protein only when phosphorylated at S389.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):PRsPA

ELK1

ETS domain-containing protein Elk-1

ELK1 ;
ETS domain-containing protein Elk-1

This gene is a member of the Ets family of transcription factors and of the ternary complex factor (TCF) subfamily. Proteins of the TCF subfamily form a ternary complex by binding to the the serum response factor and the serum response element in the promoter of the c-fos proto-oncogene. The protein encoded by this gene is a nuclear target for the ras-raf-MAPK signaling cascade. This gene produces multiple isoforms by using alternative translational start codons and by alternative splicing. Related pseudogenes have been identified on chromosomes 7 and 14. [provided by RefSeq, Mar 2012],

Function:Stimulates transcription. Binds to purine-rich DNA sequences. Can form a ternary complex with the serum response factor and the ETS and SRF motifs of the fos serum response element.,PTM:On mitogenic stimulation, phosphorylated on C-terminal serine and threonine residues by MAPK1. Ser-383 and Ser-389 are the preferred sites for MAPK1. In vitro, phosphorylation by MAPK1 potentiates ternary complex formation with the serum responses factors, SRE and SRF. Phosphorylation leads to loss of sumoylation and restores transcriptional activator activity.,PTM:Sumoylation represses transcriptional activator activity as it results in recruitment of HDAC2 to target gene promoters which leads to decreased histone acetylation and reduced transactivator activity. It also regulates nuclear retention.,similarity:Belongs to the ETS family.,similarity:Contains 1 ETS DNA-binding domain.,subunit:Interacts in its sumoylated form with PIAS2/PIASX which enhances its transcriptional activator activity.,tissue specificity:Lung and testis.,

Nucleus.

>>MAPK signaling pathway ;
>>ErbB signaling pathway ;
>>Ras signaling pathway ;
>>Focal adhesion ;
>>Insulin signaling pathway ;
>>GnRH signaling pathway ;
>>Oxytocin signaling pathway ;
>>Leishmaniasis ;
>>Hepatitis B ;
>>Human cytomegalovirus infection ;
>>Human T-cell leukemia virus 1 infection ;
>>Pathways in cancer ;
>>Proteoglycans in cancer ;
>>Endometrial cancer ;
>>Hepatocellular carcinoma