Catalog: YP0070
Size
Price
Status
Qty.
200μL
$600.00
In stock
0
100μL
$340.00
In stock
0
50μL
$190.00
In stock
0
Add to cart
Collected
Collect
Main Information
Target
Cofilin
Host Species
Rabbit
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ELISA
MW
19kD (Observed)
Conjugate/Modification
Phospho
Detailed Information
Recommended Dilution Ratio
WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:10000; IF 1:50-200
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Specificity
Phospho-Cofilin (S3) Polyclonal Antibody detects endogenous levels of Cofilin protein only when phosphorylated at S3.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):MAsGV
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage
-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
1 mg/ml
MW(Observed)
19kD
Modification
Phospho
Clonality
Polyclonal
Isotype
IgG
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Antigen&Target Information
Immunogen:
The antiserum was produced against synthesized peptide derived from human Cofilin around the phosphorylation site of Ser3. AA range:1-50
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Specificity:
Phospho-Cofilin (S3) Polyclonal Antibody detects endogenous levels of Cofilin protein only when phosphorylated at S3.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):MAsGV
show all
Gene Name:
CFL1
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Protein Name:
Cofilin-1
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Other Name:
CFL1 ;
CFL ;
Cofilin-1 ;
18 kDa phosphoprotein ;
p18 ;
Cofilin ;
non-muscle isoform
CFL ;
Cofilin-1 ;
18 kDa phosphoprotein ;
p18 ;
Cofilin ;
non-muscle isoform
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Database Link:
Background:
cofilin 1(CFL1) Homo sapiens The protein encoded by this gene can polymerize and depolymerize F-actin and G-actin in a pH-dependent manner. Increased phosphorylation of this protein by LIM kinase aids in Rho-induced reorganization of the actin cytoskeleton. Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.[supplied by OMIM, Apr 2004],
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Function:
Function:Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods.,online information:Cofilin entry,PTM:Phosphorylated on Ser-3 in resting cells.,similarity:Belongs to the actin-binding proteins ADF family.,similarity:Contains 1 ADF-H domain.,subcellular location:Almost completely in nucleus in cells exposed to heat shock or 10% dimethyl sulfoxide.,tissue specificity:Widely distributed in various tissues.,
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Cellular Localization:
Nucleus matrix . Cytoplasm, cytoskeleton . Cell projection, ruffle membrane ; Peripheral membrane protein ; Cytoplasmic side . Cell projection, lamellipodium membrane ; Peripheral membrane protein ; Cytoplasmic side . Cell projection, lamellipodium . Cell projection, growth cone . Cell projection, axon . Colocalizes with the actin cytoskeleton in membrane ruffles and lamellipodia. Detected at the cleavage furrow and contractile ring during cytokinesis. Almost completely in nucleus in cells exposed to heat shock or 10% dimethyl sulfoxide.
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Tissue Expression:
Widely distributed in various tissues.
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Research Areas:
>>Axon guidance ;
>>Fc gamma R-mediated phagocytosis ;
>>Regulation of actin cytoskeleton ;
>>Pertussis ;
>>Human immunodeficiency virus 1 infection
>>Fc gamma R-mediated phagocytosis ;
>>Regulation of actin cytoskeleton ;
>>Pertussis ;
>>Human immunodeficiency virus 1 infection
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Signaling Pathway
Cellular Processes >> Cell growth and death >> Necroptosis
Cellular Processes >> Cellular community - eukaryotes >> Signaling pathways regulating pluripotency of stem cells
Organismal Systems >> Immune system >> Toll-like receptor signaling pathway
Organismal Systems >> Immune system >> NOD-like receptor signaling pathway
Organismal Systems >> Immune system >> Th1 and Th2 cell differentiation
Organismal Systems >> Immune system >> Th17 cell differentiation
Organismal Systems >> Development and regeneration >> Osteoclast differentiation
Human Diseases >> Cancer: overview >> Pathways in cancer
Human Diseases >> Cancer: overview >> PD-L1 expression and PD-1 checkpoint pathway in cancer
Human Diseases >> Cancer: specific types >> Pancreatic cancer
Environmental Information Processing >> Signal transduction >> JAK-STAT signaling pathway
Environmental Information Processing >> Signal transduction >> PI3K-Akt signaling pathway
Reference Citation({{totalcount}})
Catalog: YP0070
Size
Price
Status
Qty.
200μL
$600.00
In stock
0
100μL
$340.00
In stock
0
50μL
$190.00
In stock
0
Add to cart
Collected
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