Catalog: YM3612
Size
Price
Status
Qty.
200μL
$450.00
3 weeks
0
100μL
$280.00
3 weeks
0
40μL
$160.00
3 weeks
0
Add to cart
Collected
Collect
Main Information
Target
Actinin-α1
Host Species
Mouse
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
MW
103kD (Observed)
Conjugate/Modification
Unmodified
Detailed Information
Recommended Dilution Ratio
IHC 1:200-400; IF/ICC1:200-400; WB 1:500-2000
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Specificity
a-actinin Monoclonal Antibody(4A12) detects endogenous levels of ACTN1
Purification
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Storage
-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
1 mg/ml
MW(Observed)
103kD
Modification
Unmodified
Clonality
Monoclonal
Clone Number
4A12
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Antigen&Target Information
Immunogen:
Recombinant Protein of a-actinin of ACTN1
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Specificity:
a-actinin Monoclonal Antibody(4A12) detects endogenous levels of ACTN1
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Gene Name:
ACTN1
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Protein Name:
ACTN1
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Other Name:
ACTN1
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Background:
Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008],
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Function:
Function:F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.,similarity:Belongs to the alpha-actinin family.,similarity:Contains 1 actin-binding domain.,similarity:Contains 2 CH (calponin-homology) domains.,similarity:Contains 2 EF-hand domains.,similarity:Contains 4 spectrin repeats.,subcellular location:Colocalizes with MYOZ2 and PPP3CA at the Z-line of heart and skeletal muscle.,subunit:Homodimer; antiparallel. Interacts with DDN, MYOZ2, PDLIM2, TTID and LPP.,
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Cellular Localization:
Cytoplasm, cytoskeleton . Cytoplasm, myofibril, sarcomere, Z line . Cell membrane . Cell junction . Cell projection, ruffle . Colocalizes with MYOZ2 and PPP3CA at the Z-line of heart and skeletal muscle. Colocalizes with PSD in membrane ruffles and central reticular structures. .
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Tissue Expression:
Research Areas:
>>Focal adhesion ;
>>Adherens junction ;
>>Tight junction ;
>>Leukocyte transendothelial migration ;
>>Regulation of actin cytoskeleton ;
>>Shigellosis ;
>>Amoebiasis ;
>>Viral carcinogenesis ;
>>Systemic lupus erythematosus
>>Adherens junction ;
>>Tight junction ;
>>Leukocyte transendothelial migration ;
>>Regulation of actin cytoskeleton ;
>>Shigellosis ;
>>Amoebiasis ;
>>Viral carcinogenesis ;
>>Systemic lupus erythematosus
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Signaling Pathway
Cellular Processes >> Cellular community - eukaryotes >> Focal adhesion
Cellular Processes >> Cellular community - eukaryotes >> Adherens junction
Cellular Processes >> Cellular community - eukaryotes >> Tight junction
Cellular Processes >> Cell motility >> Regulation of actin cytoskeleton
Organismal Systems >> Immune system >> Leukocyte transendothelial migration
Human Diseases >> Immune disease >> Systemic lupus erythematosus
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Catalog: YM3612
Size
Price
Status
Qty.
200μL
$450.00
3 weeks
0
100μL
$280.00
3 weeks
0
40μL
$160.00
3 weeks
0
Add to cart
Collected
Collect
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