Catalog: YP1159
Size
Price
Status
Qty.
200μL
$600.00
In stock
0
100μL
$340.00
In stock
0
50μL
$190.00
In stock
0
Add to cart
Collected
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Main Information
Target
NIFK
Host Species
Rabbit
Reactivity
Human, Mouse
Applications
IHC, IF, ELISA
MW
34kD (Calculated)
Conjugate/Modification
Phospho
Detailed Information
Recommended Dilution Ratio
IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:40000; Not yet tested in other applications.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Specificity
Phospho-NIFK (T234) Polyclonal Antibody detects endogenous levels of NIFK protein only when phosphorylated at T234.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):GPtPV
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage
-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
1 mg/ml
MW(Calculated)
34kD
Modification
Phospho
Clonality
Polyclonal
Isotype
IgG
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Antigen&Target Information
Immunogen:
The antiserum was produced against synthesized peptide derived from human NIFK around the phosphorylation site of Thr234. AA range:200-249
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Specificity:
Phospho-NIFK (T234) Polyclonal Antibody detects endogenous levels of NIFK protein only when phosphorylated at T234.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):GPtPV
show all
Gene Name:
MKI67IP
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Protein Name:
MKI67 FHA domain-interacting nucleolar phosphoprotein
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Other Name:
MKI67IP ;
NIFK ;
NOPP34 ;
MKI67 FHA domain-interacting nucleolar phosphoprotein ;
Nucleolar phosphoprotein Nopp34 ;
Nucleolar protein interacting with the FHA domain of pKI-67 ;
hNIFK
NIFK ;
NOPP34 ;
MKI67 FHA domain-interacting nucleolar phosphoprotein ;
Nucleolar phosphoprotein Nopp34 ;
Nucleolar protein interacting with the FHA domain of pKI-67 ;
hNIFK
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Background:
This gene encodes a protein that interacts with the forkhead-associated domain of the Ki-67 antigen. The encoded protein may bind RNA and may play a role in mitosis and cell cycle progression. Multiple pseudogenes exist on chromosomes 5, 10, 12, 15, and 19.[provided by RefSeq, Jan 2009],
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Function:
PTM:Sequentially phosphorylated on Thr-238, Thr-234 and Ser-230. Thr-234 is phosphorylated only when Thr-238 is phosphorylated. Likewise, phosphorylation at Ser-230 requires that Thr-234 and Thr-238 are phosphorylated. Phosphorylation enhances MKI67 binding.,similarity:Contains 1 RRM (RNA recognition motif) domain.,subcellular location:Localizes to mitotic chromosomes in conjunction with MKI67.,subunit:Binds to the FHA domain of MKI67; this interaction is enhanced in mitosis.,
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Cellular Localization:
Nucleus, nucleolus. Chromosome. Localizes to mitotic chromosomes in conjunction with MKI67.
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Tissue Expression:
Brain,Cervix carcinoma,Epithelium,Lung,Plac
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Reference Citation({{totalcount}})
Catalog: YP1159
Size
Price
Status
Qty.
200μL
$600.00
In stock
0
100μL
$340.00
In stock
0
50μL
$190.00
In stock
0
Add to cart
Collected
Collect
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