Catalog: YP0820
Size
Price
Status
Qty.
200μL
$600.00
In stock
0
100μL
$340.00
In stock
0
50μL
$190.00
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Main Information
Target
MAPKAPK5 Phospho Thr182
Host Species
Rabbit
Reactivity
Human, Mouse
Applications
WB, IHC, IF, ELISA
MW
60kD (Observed)
Conjugate/Modification
Phospho
Detailed Information
Recommended Dilution Ratio
WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:5000; IF 1:50-200
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Specificity
Phospho-PRAK (T182) Polyclonal Antibody detects endogenous levels of PRAK protein only when phosphorylated at T182.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):LMtPQ
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage
-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
1 mg/ml
MW(Observed)
60kD
Modification
Phospho
Clonality
Polyclonal
Isotype
IgG
Related Products
Primary Antibodies
MAPKAPK5 Rabbit pAb
YT3848
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Primary Antibodies
MAPKAPK5 (Phospho Thr182) Rabbit pAb
YP0820
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ELISA Kits
Total MAPKAPK5 Cell-Based Colorimetric ELISA Kit
KA4169C
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ELISA Kits
MAPKAPK5 (Phospho Thr182) Cell-Based Colorimetric ELISA Kit
KA1752C
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Antigen&Target Information
Immunogen:
The antiserum was produced against synthesized peptide derived from human MAPKAPK5 around the phosphorylation site of Thr182. AA range:148-197
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Specificity:
Phospho-PRAK (T182) Polyclonal Antibody detects endogenous levels of PRAK protein only when phosphorylated at T182.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):LMtPQ
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Gene Name:
MAPKAPK5
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Protein Name:
MAP kinase-activated protein kinase 5
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Other Name:
MAPKAPK5 ;
PRAK ;
MAP kinase-activated protein kinase 5 ;
MAPK-activated protein kinase 5 ;
MAPKAP kinase 5 ;
MAPKAP-K5 ;
MAPKAPK-5 ;
MK-5 ;
MK5 ;
p38-regulated/activated protein kinase ;
PRAK
PRAK ;
MAP kinase-activated protein kinase 5 ;
MAPK-activated protein kinase 5 ;
MAPKAP kinase 5 ;
MAPKAP-K5 ;
MAPKAPK-5 ;
MK-5 ;
MK5 ;
p38-regulated/activated protein kinase ;
PRAK
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Background:
The protein encoded by this gene is a tumor suppressor and member of the serine/threonine kinase family. In response to cellular stress and proinflammatory cytokines, this kinase is activated through its phosphorylation by MAP kinases including MAPK1/ERK, MAPK14/p38-alpha, and MAPK11/p38-beta. The encoded protein is found in the nucleus but translocates to the cytoplasm upon phosphorylation and activation. This kinase phosphorylates heat shock protein HSP27 at its physiologically relevant sites. Two alternately spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Nov 2012],
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Function:
Catalytic activity:ATP + a protein = ADP + a phosphoprotein.,enzyme regulation:p38 alpha and beta-dependent phosphorylation increases its activity. Activated by stress-related extracellular stimuli; such as H(2)O(2), arsenite, anisomycin TNF alpha and also PMA and the calcium ionophore A23187; but to a lesser extent. In vitro, activated by SQSTM1.,Function:Mediates stress-induced small heat shock protein 27 phosphorylation.,PTM:Phosphorylated on Thr-182; which is the regulatory phosphorylation site and is located on the T-loop/loop 12.,similarity:Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.,similarity:Contains 1 protein kinase domain.,subcellular location:Also observed in the nucleus.,subunit:Interacts with SQSTM1.,tissue specificity:Expressed ubiquitously.,
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Cellular Localization:
Cytoplasm. Nucleus. Translocates to the cytoplasm following phosphorylation and activation. Interaction with ERK3/MAPK6 or ERK4/MAPK4 and phosphorylation at Thr-182, activates the protein kinase activity, followed by translocation to the cytoplasm. Phosphorylation by PKA/PRKACA at Ser-115 also induces nuclear export.
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Tissue Expression:
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Research Areas:
>>MAPK signaling pathway
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Signaling Pathway
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Catalog: YP0820
Size
Price
Status
Qty.
200μL
$600.00
In stock
0
100μL
$340.00
In stock
0
50μL
$190.00
In stock
0
Add to cart
Collected
Collect
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