Catalog: YP0548
Size
Price
Status
Qty.
200μL
$600.00
In stock
0
100μL
$340.00
In stock
0
50μL
$190.00
In stock
0
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Main Information
Target
DAPK3 Phospho Thr265
Host Species
Rabbit
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ELISA
MW
52kD (Observed)
Conjugate/Modification
Phospho
Detailed Information
Recommended Dilution Ratio
WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:10000; Not yet tested in other applications.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Specificity
Phospho-DAPK3 (T265) Polyclonal Antibody detects endogenous levels of DAPK3 protein only when phosphorylated at T265.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):RMtIA
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage
-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
1 mg/ml
MW(Observed)
52kD
Modification
Phospho
Clonality
Polyclonal
Isotype
IgG
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Antigen&Target Information
Immunogen:
The antiserum was produced against synthesized peptide derived from human DAPK3 around the phosphorylation site of Thr265. AA range:241-290
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Specificity:
Phospho-DAPK3 (T265) Polyclonal Antibody detects endogenous levels of DAPK3 protein only when phosphorylated at T265.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):RMtIA
show all
Gene Name:
DAPK3
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Protein Name:
Death-associated protein kinase 3
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Other Name:
DAPK3 ;
ZIPK ;
Death-associated protein kinase 3 ;
DAP kinase 3 ;
DAP-like kinase ;
Dlk ;
MYPT1 kinase ;
Zipper-interacting protein kinase ;
ZIP-kinase
ZIPK ;
Death-associated protein kinase 3 ;
DAP kinase 3 ;
DAP-like kinase ;
Dlk ;
MYPT1 kinase ;
Zipper-interacting protein kinase ;
ZIP-kinase
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Database Link:
Background:
Death-associated protein kinase 3 (DAPK3) induces morphological changes in apoptosis when overexpressed in mammalian cells. These results suggest that DAPK3 may play a role in the induction of apoptosis. [provided by RefSeq, Jul 2008],
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Function:
Catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,Function:Serine/threonine kinase which acts as a positive regulator of apoptosis. Phosphorylates histone H3 on 'Thr-11' at centromeres during mitosis.,similarity:Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. DAP kinase subfamily.,similarity:Contains 1 protein kinase domain.,subcellular location:Relocates to the cytoplasm on binding PAWR where the complex appears to interact with actin filaments (By similarity). Associates to centromeres from prophase to anaphase.,subunit:Homodimer or forms heterodimers with ATF4. Both interactions require an intact leucine zipper domain and oligomerization is required for full enzymatic activity. Also binds to DAXX and PAWR, possibly in a ternary complex which plays a role in caspase activation. Interacts with AATF and CDC5L.,
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Cellular Localization:
Nucleus . Cytoplasm . Predominantly localizes to the cytoplasm but can shuttle between the nucleus and cytoplasm; cytoplasmic localization is promoted by phosphorylation at Thr-299 and involves Rho/Rock signaling. .; [Isoform 1]: Nucleus . Cytoplasm .; [Isoform 2]: Nucleus . Cytoplasm .
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Tissue Expression:
Research Areas:
>>Autophagy - animal ;
>>Pathways in cancer ;
>>Bladder cancer
>>Pathways in cancer ;
>>Bladder cancer
show all
Signaling Pathway
Reference Citation({{totalcount}})
Catalog: YP0548
Size
Price
Status
Qty.
200μL
$600.00
In stock
0
100μL
$340.00
In stock
0
50μL
$190.00
In stock
0
Add to cart
Collected
Collect
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