Purified recombinant human PA28γ protein fragments expressed in E.coli.

PA28γ Monoclonal Antibody detects endogenous levels of PA28γ protein.

PSME3

Proteasome activator complex subunit 3

PSME3 ;
Proteasome activator complex subunit 3 ;
11S regulator complex subunit gamma ;
REG-gamma ;
Activator of multicatalytic protease subunit 3 ;
Ki nuclear autoantigen ;
Proteasome activator 28 subunit gamma ;
PA28g ;
PA28gamma

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) o

Disease:Sera from patients with systemic lupus erythematosus often contain antibodies that react with the Ki antigen.,Domain:The C-terminal sequences affect heptamer stability and proteasome affinity.,Function:Subunit of the 11S REG-gamma (also called PA28-gamma) proteasome regulator, a donut-shaped homoheptamer which associates with the proteasome. 11S REG-gamma activates the trypsin-like catalytic subunit of the proteasome but inhibits the chymotrypsin-like and postglutamyl-preferring (PGPH) subunits. Facilitates the MDM2-TP53/p53 interaction which promotes ubiquitination- and MDM2-dependent proteasomal degradation of TP53/p53, limiting its accumulation and resulting in inhibited apoptosis after DNA damage. May also be involved in cell cycle regulation.,induction:Up-regulated in thyroid carcinoma cells.,PTM:Phosphorylated by MAP3K3.,similarity:Belongs to the PA28 family.,subcellular location:Localizes to the cytoplasm during mitosis following nuclear envelope breakdown at this distinct stage of the cell cycle which allows its interaction with MAP3K3 kinase.,subunit:Homoheptamer; the stability of the heptamer is essential for the specific activation of the trypsine-like subunit and inhibition of the chymotrypsin-like and postglutamyl-preferring (PGPH) subunits of the proteasome. Interacts with TP53/p53 and MDM2. Interacts with MAP3K3 (By similarity). Associates with the proteasome.,

Nucleus . Cytoplasm . Localizes to the cytoplasm during mitosis following nuclear envelope breakdown at this distinct stage of the cell cycle which allows its interaction with MAP3K3 kinase. .

B-cell,Embryonic kidney,Fetal brain,Human endometrium carcinoma cell line,L

>>Proteasome ;
>>Antigen processing and presentation ;
>>Hepatitis C