The antiserum was produced against synthesized peptide derived from human eNOS. AA range:1145-1194

NOS3 Polyclonal Antibody detects endogenous levels of NOS3 protein.

NOS3

Nitric oxide synthase endothelial

NOS3 ;
Nitric oxide synthase ;
endothelial ;
Constitutive NOS ;
cNOS ;
EC-NOS ;
Endothelial NOS ;
eNOS ;
NOS type III ;
NOSIII

Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes , including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq , May 2009] ,

Catalytic activity:L-arginine + n NADPH + n H (+) + m O (2) = citrulline + nitric oxide + n NADP (+) . ,cofactor:Binds 1 FAD. ,cofactor:Binds 1 FMN. ,cofactor:Heme group. ,cofactor:Tetrahydrobiopterin (BH4) . May stabilize the dimeric form of the enzyme. ,enzyme regulation:Stimulated by calcium/calmodulin. Inhibited by NOSIP and NOSTRIN. ,Function:Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF) -induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. ,online information:Nitric oxide synthase entry ,polymorphism:Variation in NOS3 seem to be associated with susceptibility to coronary spasm. ,similarity:Belongs to the NOS family. ,similarity:Contains 1 FAD-binding FR-type domain. ,similarity:Contains 1 flavodoxin-like domain. ,subcellular location:Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle; which is favored by interaction with NOSIP and results in a reduced enzymatic activity. ,subunit:Homodimer. Interacts with NOSIP and NOSTRIN. ,tissue specificity:Platelets , placenta , liver and kidney. ,

Cell membrane. Membrane , caveola. Cytoplasm , cytoskeleton. Golgi apparatus. Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle; which is favored by interaction with NOSIP and results in a reduced enzymatic activity.

>>Arginine biosynthesis ;
>>Arginine and proline metabolism ;
>>Metabolic pathways ;
>>Calcium signaling pathway ;
>>cGMP-PKG signaling pathway ;
>>HIF-1 signaling pathway ;
>>Sphingolipid signaling pathway ;
>>PI3K-Akt signaling pathway ;
>>VEGF signaling pathway ;
>>Apelin signaling pathway ;
>>Platelet activation ;
>>Estrogen signaling pathway ;
>>Oxytocin signaling pathway ;
>>Relaxin signaling pathway ;
>>Insulin resistance ;
>>AGE-RAGE signaling pathway in diabetic complications ;
>>Diabetic cardiomyopathy ;
>>Lipid and atherosclerosis ;
>>Fluid shear stress and atherosclerosis