• Target：
• GADD34

• Fields：
• >>Protein processing in endoplasmic reticulum

• Gene Name：
• PPP1R15A GADD34

• Protein Name：
• Protein phosphatase 1 regulatory subunit 15A (Growth arrest and DNA damage-inducible protein GADD34) (Myeloid differentiation primary response protein MyD116 homolog)

• Human Gene Id：
• 23645

• Human Swiss Prot No：
• O75807

• Mouse Gene Id：
• 17872

• Mouse Swiss Prot No：
• P17564

• Immunogen：
• Synthetic peptide from human protein at AA range: 430-490

• Specificity：
• The antibody detects endogenous GADD34

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500-2000, ELISA 1:10000-20000

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• Protein phosphatase 1 regulatory subunit 15A (Growth arrest and DNA damage-inducible protein GADD34) (Myeloid differentiation primary response protein MyD116 homolog)

• Observed Band(KD)：
• 73kD

• Background：
• This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The induction of this gene by ionizing radiation occurs in certain cell lines regardless of p53 status, and its protein response is correlated with apoptosis following ionizing radiation. [provided by RefSeq, Jul 2008],

• Function：
• function:Recruits the serine/threonine-protein phosphatase PP1 to dephosphorylate the translation initiation factor eIF-2A/EIF2S1, thereby reversing the shut-off of protein synthesis initiated by stress-inducible kinases and facilitating recovery of cells from stress. Down-regulates the TGF-beta signaling pathway by promoting dephosphorylation of TGFB1 by PP1. May promote apoptosis by inducing TP53 phosphorylation on 'Ser-15'.,induction:By methyl methanesulfonate and ionizing irradiation. By IL24 in melanoma cells; which induces apoptosis.,miscellaneous:The phosphatase activity of the PPP1R15A-PP1 complex toward EIF2S1 is specifically inhibited by Salubrinal, a drug that protects cells from endoplasmic reticulum stress.,PTM:Phosphorylated on tyrosine by LYN; which impairs its antiproliferative activity.,similarity:Belongs to the PPP1R15 family.,subunit:Interacts with PCNA (By similarity)

• Subcellular Location：
• Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side . Mitochondrion outer membrane; Peripheral membrane protein; Cytoplasmic side . Associates with membranes via an N-terminal amphipathic intramembrane region. .

• Expression：
• Brain,

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