GADD34 Polyclonal Antibody

    • Catalog No.:YT5900
    • Applications:WB,ELISA
    • Reactivity:Human
      • Gene Name:
      • PPP1R15A GADD34
      • Protein Name:
      • Protein phosphatase 1 regulatory subunit 15A (Growth arrest and DNA damage-inducible protein GADD34) (Myeloid differentiation primary response protein MyD116 homolog)
      • Human Swiss Prot No:
      • O75807
      • Mouse Swiss Prot No:
      • P17564
      • Immunogen:
      • Synthetic peptide from human protein at AA range: 430-490
      • Specificity:
      • The antibody detects endogenous GADD34
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Rabbit
      • Dilution:
      • WB 1:500-2000, ELISA 1:10000-20000
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -20°C/1 year
      • Other Name:
      • Protein phosphatase 1 regulatory subunit 15A (Growth arrest and DNA damage-inducible protein GADD34) (Myeloid differentiation primary response protein MyD116 homolog)
      • Observed Band(KD):
      • 73
      • Background:
      • protein phosphatase 1 regulatory subunit 15A(PPP1R15A) Homo sapiens This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The induction of this gene by ionizing radiation occurs in certain cell lines regardless of p53 status, and its protein response is correlated with apoptosis following ionizing radiation. [provided by RefSeq, Jul 2008],
      • Function:
      • function:Recruits the serine/threonine-protein phosphatase PP1 to dephosphorylate the translation initiation factor eIF-2A/EIF2S1, thereby reversing the shut-off of protein synthesis initiated by stress-inducible kinases and facilitating recovery of cells from stress. Down-regulates the TGF-beta signaling pathway by promoting dephosphorylation of TGFB1 by PP1. May promote apoptosis by inducing TP53 phosphorylation on 'Ser-15'.,induction:By methyl methanesulfonate and ionizing irradiation. By IL24 in melanoma cells; which induces apoptosis.,miscellaneous:The phosphatase activity of the PPP1R15A-PP1 complex toward EIF2S1 is specifically inhibited by Salubrinal, a drug that protects cells from endoplasmic reticulum stress.,PTM:Phosphorylated on tyrosine by LYN; which impairs its antiproliferative activity.,similarity:Belongs to the PPP1R15 family.,subunit:Interacts with PCNA (By similarity)
      • Subcellular Location:
      • protein phosphatase type 1 complex,cytoplasm,mitochondrion,mitochondrial outer membrane,endoplasmic reticulum,endoplasmic reticulum membrane,Golgi apparatus,cytosol,membrane,
      • Expression:
      • Brain,
      • Products Images
      • Western blot analysis of MOUSE-BRAIN lysate, antibody was diluted at 1000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000