Rab 5A Polyclonal Antibody

    • Catalog No.:YT5456
    • Applications:WB;IHC;IF;ELISA
    • Reactivity:Human;Mouse;Rat
      • Target:
      • Rab 5A
      • Fields:
      • >>Ras signaling pathway;>>Endocytosis;>>Phagosome;>>Vasopressin-regulated water reabsorption;>>Amyotrophic lateral sclerosis;>>Pathways of neurodegeneration - multiple diseases;>>Salmonella infection;>>Amoebiasis;>>Tuberculosis
      • Gene Name:
      • RAB5A
      • Protein Name:
      • Ras-related protein Rab-5A
      • Human Gene Id:
      • 5868
      • Human Swiss Prot No:
      • P20339
      • Mouse Swiss Prot No:
      • Q9CQD1
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from the Internal region of human RAB5A. AA range:71-120
      • Specificity:
      • Rab 5A Polyclonal Antibody detects endogenous levels of Rab 5A protein.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Polyclonal, Rabbit,IgG
      • Dilution:
      • WB 1:500 - 1:2000. IHC-p: 1:100-1:300. ELISA: 1:20000.. IF 1:50-200
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Other Name:
      • RAB5A;RAB5;Ras-related protein Rab-5A
      • Observed Band(KD):
      • 24kD
      • Background:
      • enzyme regulation:Regulated by guanine nucleotide exchange factors (GEFs) which promote the exchange of bound GDP for free GTP.,function:Required for the fusion of plasma membranes and early endosomes.,similarity:Belongs to the small GTPase superfamily. Rab family.,subcellular location:Enriched in stage I melanosomes.,subunit:Binds EEA1. Interacts with RIN1 and GAPVD1, which regulate its pathway, probably by acting as a GEF. Interacts with ALS2CL, UNC84B, ZFYVE20 and RUFY1. Interacts with SGSM1 and SGSM3.,
      • Function:
      • enzyme regulation:Regulated by guanine nucleotide exchange factors (GEFs) which promote the exchange of bound GDP for free GTP.,function:Required for the fusion of plasma membranes and early endosomes.,similarity:Belongs to the small GTPase superfamily. Rab family.,subcellular location:Enriched in stage I melanosomes.,subunit:Binds EEA1. Interacts with RIN1 and GAPVD1, which regulate its pathway, probably by acting as a GEF. Interacts with ALS2CL, UNC84B, ZFYVE20 and RUFY1. Interacts with SGSM1 and SGSM3.,
      • Subcellular Location:
      • Cell membrane ; Lipid-anchor ; Cytoplasmic side . Early endosome membrane ; Lipid-anchor . Melanosome . Cytoplasmic vesicle . Cell projection, ruffle . Membrane . Cytoplasm, cytosol . Cytoplasmic vesicle, phagosome membrane . Endosome membrane . Enriched in stage I melanosomes (PubMed:17081065). Alternates between membrane-bound and cytosolic forms (Probable). .
      • Expression:
      • Brain,Cervix,Cervix carcinoma,Placenta,
      • Products Images
      • Western Blot analysis of HeLa cells using Rab 5A Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
      • Immunohistochemical analysis of paraffin-embedded human-lung, antibody was diluted at 1:100
      • Western blot analysis of lysate from HeLa cells, using RAB5A Antibody.