PDGF-D Polyclonal Antibody

    • Catalog No.:YT5299
    • Applications:WB,IHC-p,IF(paraffin section),ELISA
    • Reactivity:Human
      • Gene Name:
      • PDGFD
      • Protein Name:
      • Platelet-derived growth factor D
      • Human Swiss Prot No:
      • Q9GZP0
      • Mouse Swiss Prot No:
      • Q925I7
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from the C-terminal region of human PDGFD. AA range:311-360
      • Specificity:
      • PDGF-D Polyclonal Antibody detects endogenous levels of PDGF-D protein.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Rabbit
      • Dilution:
      • IF(paraffin section) 1:50-200 Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -20°C/1 year
      • Other Name:
      • PDGFD; IEGF; SCDGFB; MSTP036; Platelet-derived growth factor D; PDGF-D; Iris-expressed growth factor; Spinal cord-derived growth factor B; SCDGF-B
      • MolecularWeight(Da):
      • 42848
      • Observed Band(KD):
      • 42
      • Background:
      • platelet derived growth factor D(PDGFD) Homo sapiens The protein encoded by this gene is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a core motif of eight cysteines, seven of which are found in this factor. This gene product only forms homodimers and, therefore, does not dimerize with the other three family members. It differs from alpha and beta members of this family in having an unusual N-terminal domain, the CUB domain. Two splice variants have been identified for this gene. [provided by RefSeq, Jul 2008],
      • Function:
      • developmental stage:Not detectable in the earliest stages of glomerulogenesis, and not detected in the metanephric blastema or surrounding cortical interstitial cells. In later stages of glomerulogenesis, localized to epithelial cells transitioning from the early developing nephrons of the comma- and S-shaped stages to the visceral epithelial cells of differentiated glomeruli. In the developing pelvis, expressed at the basement membrane of immature collecting ducts and by presumptive fibroblastic cells in the interstitium.,function:Potent mitogen for cells of mesenchymal origin. Binding of this growth factor to its affinity receptor elicits a variety of cellular responses. It is released by platelets upon wounding and plays an important role in stimulating adjacent cells to grow and thereby heals the wound. Activated by proteolytic cleavage and this active form acts as a specific ligand
      • Subcellular Location:
      • Golgi membrane,extracellular region,extracellular space,endoplasmic reticulum lumen,extracellular exosome,
      • Expression:
      • Aorta,Iris,Testis,
      • Products Images
      • Western Blot analysis of L929 cells using PDGF-D Polyclonal Antibody.. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
      • Immunohistochemical analysis of paraffin-embedded human-mammary-cancer, antibody was diluted at 1:100
      • Immunohistochemical analysis of paraffin-embedded human-lung-cancer, antibody was diluted at 1:100
      • Western blot analysis of lysate from L929 cells, using PDGFD Antibody.