MK Polyclonal Antibody

    • Catalog No.:YT5177
    • Applications:WB,IHC-p,ELISA
    • Reactivity:Human,Rat
      • Gene Name:
      • MDK
      • Protein Name:
      • Midkine
      • Human Gene Id:
      • 4192
      • Human Swiss Prot No:
      • P21741
      • Mouse Swiss Prot No:
      • P12025
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from the Internal region of human MDK. AA range:94-143
      • Specificity:
      • MK Polyclonal Antibody detects endogenous levels of MK protein.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Rabbit
      • Dilution:
      • Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -20°C/1 year
      • Other Name:
      • MDK; MK1; NEGF2; Midkine; MK; Amphiregulin-associated protein; ARAP; Midgestation and kidney protein; Neurite outgrowth-promoting factor 2; Neurite outgrowth-promoting protein
      • MolecularWeight(Da):
      • 15,585
      • Observed Band(KD):
      • 18
      • Background:
      • midkine (neurite growth-promoting factor 2)(MDK) Homo sapiens This gene encodes a member of a small family of secreted growth factors that binds heparin and responds to retinoic acid. The encoded protein promotes cell growth, migration, and angiogenesis, in particular during tumorigenesis. This gene has been targeted as a therapeutic for a variety of different disorders. Alternatively spliced transcript variants encoding multiple isoforms have been observed. [provided by RefSeq, Jul 2012],
      • Function:
      • function:Has heparin binding activity, and growth promoting activity. Involved in neointima formation after arterial injury, possibly by mediating leukocyte recruitment. Also involved in early fetal adrenal gland development.,induction:By heparin and retinoic acid.,similarity:Belongs to the pleiotrophin family.,subunit:Homodimer.,
      • Subcellular Location:
      • extracellular region,cytoplasm,cell projection,
      • Expression:
      • Fetal brain,Kidney,Plasma,Uterus,
      • Products Images
      • Western Blot analysis of SW480, MCF7, PC-3, Raji, K562, mouse eye, mouse kidney cells using MK Polyclonal Antibody.. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
      • Immunohistochemical analysis of paraffin-embedded human-brain, antibody was diluted at 1:100
      • Immunohistochemical analysis of paraffin-embedded rat-brain, antibody was diluted at 1:100
      • Immunohistochemical analysis of paraffin-embedded rat-brain, antibody was diluted at 1:100
      • Immunohistochemical analysis of paraffin-embedded rat-brain, antibody was diluted at 1:100
      • Immunohistochemical analysis of paraffin-embedded Human Prostate. 1, Antibody was diluted at 1:100(4°,overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
      • Immunohistochemical analysis of paraffin-embedded Human Prostate. 1, Antibody was diluted at 1:100(4°,overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
      • Immunohistochemical analysis of paraffin-embedded Human Prostate. 1, Antibody was diluted at 1:100(4°,overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
      • Western blot analysis of lysate from SW480 cells, using MDK Antibody.