TdT Polyclonal Antibody

    • Catalog No.:YT4594
    • Applications:WB;IHC;IF;ELISA
    • Reactivity:Human;Rat;Mouse;
      • Target:
      • TdT
      • Fields:
      • >>Non-homologous end-joining;>>Hematopoietic cell lineage
      • Gene Name:
      • DNTT
      • Protein Name:
      • DNA nucleotidylexotransferase
      • Human Gene Id:
      • 1791
      • Human Swiss Prot No:
      • P04053
      • Mouse Swiss Prot No:
      • P09838
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human DNTT. AA range:381-430
      • Specificity:
      • TdT Polyclonal Antibody detects endogenous levels of TdT protein.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Polyclonal, Rabbit,IgG
      • Dilution:
      • IHC-p: 100-300.WB 1:500 - 1:2000. ELISA: 1:40000.. IF 1:50-200
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Other Name:
      • DNTT;TDT;DNA nucleotidylexotransferase;Terminal addition enzyme;Terminal deoxynucleotidyltransferase;Terminal transferase
      • Observed Band(KD):
      • 60kD
      • Background:
      • This gene is a member of the DNA polymerase type-X family and encodes a template-independent DNA polymerase that catalyzes the addition of deoxynucleotides to the 3'-hydroxyl terminus of oligonucleotide primers. In vivo, the encoded protein is expressed in a restricted population of normal and malignant pre-B and pre-T lymphocytes during early differentiation, where it generates antigen receptor diversity by synthesizing non-germ line elements (N-regions) at the junctions of rearranged Ig heavy chain and T cell receptor gene segments. Alternatively spliced transcript variants encoding different isoforms of this gene have been described. [provided by RefSeq, Jul 2008],
      • Function:
      • catalytic activity:Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).,cofactor:Magnesium.,disease:Very high levels of enzyme activity have been detected in certain acute leukemic cells.,function:Template-independent DNA polymerase which catalyzes the random addition of deoxynucleoside 5'-triphosphate to the 3'-end of a DNA initiator. One of the in vivo functions of this enzyme is the addition of nucleotides at the junction (N region) of rearranged Ig heavy chain and T-cell receptor gene segments during the maturation of B- and T-cells.,similarity:Belongs to the DNA polymerase type-X family.,similarity:Contains 1 BRCT domain.,subunit:Interacts with PRP19 and DNTTIP1. Forms a ternary complex with DNTTIP2 and core histone. Released from this complex by PCNA.,
      • Subcellular Location:
      • Nucleus .
      • Expression:
      • Testis,Thymus,
      • Products Images
      • Western Blot analysis of HepG2 cells using TdT Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
      • Immunohistochemical analysis of paraffin-embedded Human Thyroid. 1, Antibody was diluted at 1:200(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
      • Immunohistochemical analysis of paraffin-embedded Human thymus. 1, Antibody was diluted at 1:200(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
      • Immunohistochemical analysis of paraffin-embedded Human thymus. 1, Antibody was diluted at 1:200(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
      • Western blot analysis of DNTT Antibody. The lane on the right is blocked with the DNTT peptide.