Stat3 Polyclonal Antibody

    • Catalog No.:YT4445
    • Applications:WB,IHC-p,IF/ICC,ELISA
    • Reactivity:Human,Mouse,Rat
      • Gene Name:
      • STAT3
      • Protein Name:
      • Signal transducer and activator of transcription 3
      • Human Gene Id:
      • 6774
      • Human Swiss Prot No:
      • P40763
      • Mouse Swiss Prot No:
      • P42227
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human STAT3. AA range:694-743
      • Specificity:
      • Stat3 Polyclonal Antibody detects endogenous levels of Stat3 protein.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Rabbit
      • Dilution:
      • Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -20°C/1 year
      • Other Name:
      • STAT3; APRF; Signal transducer and activator of transcription 3; Acute-phase response factor
      • MolecularWeight(Da):
      • 88068
      • Observed Band(KD):
      • 88
      • Background:
      • signal transducer and activator of transcription 3(STAT3) Homo sapiens The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. Mutations in this gene are associated with infantile-onset multisystem autoimmune disease and hyper
      • Function:
      • disease:Defects in STAT3 are the cause of hyperimmunoglobulin E recurrent infection syndrome autosomal dominant (AD-HIES) [MIM:147060]; also known as hyper-IgE syndrome or Job syndrome. AD-HIES is a rare disorder of immunity and connective tissue characterized by immunodeficiency, chronic eczema, recurrent Staphylococcal infections, increased serum IgE, eosinophilia, distinctive coarse facial appearance, abnormal dentition, hyperextensibility of the joints, and bone fractures.,function:Transcription factor that binds to the interleukin-6 (IL-6)-responsive elements identified in the promoters of various acute-phase protein genes. Activated by IL31 through IL31RA.,miscellaneous:Involved in the gp130-mediated signaling pathway.,online information:STAT3 entry,online information:STAT3 mutation db,PTM:Tyrosine phosphorylated in response to IL-6, IL-11, CNTF, LIF, CSF-1, EGF, PDGF, IFN-alpha an
      • Subcellular Location:
      • nuclear chromatin,nucleus,nucleoplasm,cytoplasm,mitochondrial inner membrane,cytosol,plasma membrane,RNA polymerase II transcription factor complex,
      • Expression:
      • Epithelium,Kidney,Liver,Pancreas,Placenta,
      • Products Images
      • Cai, Xiaoli, et al. "Inflammatory factor TNF-α promotes the growth of breast cancer via the positive feedback loop of TNFR1/NF-κB (and/or p38)/p-STAT3/HBXIP/TNFR1." Oncotarget 8.35 (2017): 58338.
      • Immunofluorescence analysis of Hela cell. 1,Stat3 Polyclonal Antibody(red) was diluted at 1:200(4° overnight). Caspase-8 Monoclonal Antibody(2G12)(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog:RS3611 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog:RS3208 was diluted at 1:1000(room temperature, 50min).
      • Western Blot analysis of various cells using Stat3 Polyclonal Antibody diluted at 1:2000
      • Western Blot analysis of NIH-3T3 cells using Stat3 Polyclonal Antibody diluted at 1:2000
      • The picture was kindly provided by our customer
      • Immunohistochemistry analysis of paraffin-embedded human brain tissue, using STAT3 Antibody. The picture on the right is blocked with the synthesized peptide.
      • Western blot analysis of lysates from HeLa and HT-29 cells, using STAT3 Antibody. The lane on the right is blocked with the synthesized peptide.