S-100 α Polyclonal Antibody

    • Catalog No.:YT4197
    • Applications:WB;IHC;IF;ELISA
    • Reactivity:Human;Mouse;Rat
      • Target:
      • S-100 α
      • Gene Name:
      • S100A1
      • Protein Name:
      • Protein S100-A1
      • Human Gene Id:
      • 6271
      • Human Swiss Prot No:
      • P23297
      • Mouse Swiss Prot No:
      • P56565
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human S100 A1. AA range:10-59
      • Specificity:
      • S-100 α Polyclonal Antibody detects endogenous levels of S-100 α protein.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Polyclonal, Rabbit,IgG
      • Dilution:
      • WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Other Name:
      • S100A1;S100A;Protein S100-A1;S-100 protein alpha chain;S-100 protein subunit alpha;S100 calcium-binding protein A1
      • Observed Band(KD):
      • 10kD
      • Background:
      • S100 calcium binding protein A1(S100A1) Homo sapiens The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in stimulation of Ca2+-induced Ca2+ release, inhibition of microtubule assembly, and inhibition of protein kinase C-mediated phosphorylation. Reduced expression of this protein has been implicated in cardiomyopathies. [provided by RefSeq, Jul 2008],
      • Function:
      • function:Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites.,similarity:Belongs to the S-100 family.,similarity:Contains 2 EF-hand domains.,subunit:Dimer of either two alpha chains, or two beta chains, or one alpha and one beta chain.,tissue specificity:Highly prevalent in heart. Also found in lesser quantities in skeletal muscle and brain.,
      • Subcellular Location:
      • Cytoplasm . Sarcoplasmic reticulum . Mitochondrion .
      • Expression:
      • Highly prevalent in heart (PubMed:12804600, PubMed:1384693). Also found in lesser quantities in skeletal muscle and brain (PubMed:1384693).
      • Products Images
      • Western Blot analysis of various cells using S-100 α Polyclonal Antibody
      • Immunofluorescence analysis of A549 cells, using S100 A1 Antibody. The picture on the right is blocked with the synthesized peptide.
      • Immunohistochemistry analysis of paraffin-embedded human brain tissue, using S100 A1 Antibody. The picture on the right is blocked with the synthesized peptide.
      • Western blot analysis of lysates from A549 cells, using S100 A1 Antibody. The lane on the right is blocked with the synthesized peptide.