PDGFR-β Polyclonal Antibody

    • Catalog No.:YT3639
    • Applications:WB,IHC-p,IF/ICC,ELISA
    • Reactivity:Human,Mouse,Rat
      • Gene Name:
      • PDGFRB
      • Protein Name:
      • Platelet-derived growth factor receptor beta
      • Human Gene Id:
      • 5159
      • Human Swiss Prot No:
      • P09619
      • Mouse Swiss Prot No:
      • P05622
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human PDGF Receptor beta. AA range:718-767
      • Specificity:
      • PDGFR-β Polyclonal Antibody detects endogenous levels of PDGFR-β protein.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Rabbit
      • Dilution:
      • Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -20°C/1 year
      • Other Name:
      • PDGFRB; PDGFR; PDGFR1; Platelet-derived growth factor receptor beta; PDGF-R-beta; PDGFR-beta; Beta platelet-derived growth factor receptor; Beta-type platelet-derived growth factor receptor; CD140 antigen-like family member B; Platelet-deri
      • MolecularWeight(Da):
      • 123968
      • Observed Band(KD):
      • 124
      • Background:
      • platelet derived growth factor receptor beta(PDGFRB) Homo sapiens This gene encodes a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. This gene is flanked on chromosome 5 by the genes for granulocyte-macrophage colony-stimulating factor and macrophage-colony stimulating factor receptor; all three genes may be implicated in the 5-q syndrome. A translocation between chromosomes 5 and 12, that fuses this gene to that of the translocation, ETV6, leukemia gene, results in chronic myeloproliferative disorder with eosinophilia. [provided by RefSeq, Jul 2008],
      • Function:
      • catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,disease:A chromosomal aberration involving PDGFRB is a cause in many instances of chronic myeloproliferative disorder with eosinophilia (MPE) [MIM:131440]. Translocation t(5;12) with ETV6 on chromosome 12 creating an PDGFRB-ETV6 fusion protein.,disease:A chromosomal aberration involving PDGFRB is found in a form of chronic myelomonocytic leukemia (CMML). Translocation t(5;12)(q33;p13) with EVT6/TEL. It is characterized by abnormal clonal myeloid proliferation and by progression to acute myelogenous leukemia (AML).,disease:A chromosomal aberration involving PDGFRB may be a cause of acute myelogenous leukemia. Translocation t(5;14)(q33;q32) with TRIP11. The fusion protein may be involved in clonal evolution of leukemia and eosinophilia.,disease:A chromosomal aberration involving PDGFRB may be a cause
      • Subcellular Location:
      • nucleus,cytoplasm,plasma membrane,focal adhesion,cell surface,membrane,integral component of membrane,cytoplasmic, membrane-bounded vesicle,apical plasma membrane,intrinsic component of plasma membrane,cytoplasmic v
      • Expression:
      • Brain,Spleen,
      • Products Images
      • Western blot analysis of lysates from 1) Hela, 2) K562, 3) KB cells, (Green) primary antibody was diluted at 1:1000, 4°over night, secondary antibody(cat:RS23920)was diluted at 1:10000, 37° 1hour. (Red) GAPDH Monoclonal Antibody(2B8) (cat:YM3029) antibody was diluted at 1:5000 as loading control, 4° over night,secondary antibody(cat:RS23710)was diluted at 1:10000, 37° 1hour.
      • Western Blot analysis of various cells using PDGFR-β Polyclonal Antibody diluted at 1:1000
      • Immunofluorescence analysis of HepG2 cells, using PDGF Receptor beta Antibody. The picture on the right is blocked with the synthesized peptide.
      • Immunohistochemistry analysis of paraffin-embedded human brain tissue, using PDGF Receptor beta Antibody. The picture on the right is blocked with the synthesized peptide.
      • Western blot analysis of lysates from LOVO cells, using PDGF Receptor beta Antibody. The lane on the right is blocked with the synthesized peptide.
      • Western blot analysis of lysates from 1) Hela, 2) K562, 3) KB cells, (Green) primary antibody was diluted at 1:1000, 4°over night, secondary antibody(cat:RS23920)was diluted at 1:10000, 37° 1hour. (Red) GAPDH Monoclonal Antibody(2B8) (cat:YM3029) antibody was diluted at 1:5000 as loading control, 4° over night,secondary antibody(cat:RS23710)was diluted at 1:10000, 37° 1hour.