p300 Polyclonal Antibody

    • Catalog No.:YT3507
    • Applications:WB,IHC-p,IF(paraffin section),ELISA
    • Reactivity:Human,Mouse,Rat
      • Gene Name:
      • EP300
      • Protein Name:
      • Histone acetyltransferase p300
      • Human Gene Id:
      • 2033
      • Human Swiss Prot No:
      • Q09472
      • Mouse Swiss Prot No:
      • B2RWS6
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human p300. AA range:55-104
      • Specificity:
      • p300 Polyclonal Antibody detects endogenous levels of p300 protein.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Rabbit
      • Dilution:
      • Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -20°C/1 year
      • Other Name:
      • EP300; P300; Histone acetyltransferase p300; p300 HAT; E1A-associated protein p300
      • MolecularWeight(Da):
      • 264144
      • Observed Band(KD):
      • 260
      • Background:
      • E1A binding protein p300(EP300) Homo sapiens This gene encodes the adenovirus E1A-associated cellular p300 transcriptional co-activator protein. It functions as histone acetyltransferase that regulates transcription via chromatin remodeling and is important in the processes of cell proliferation and differentiation. It mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. This gene has also been identified as a co-activator of HIF1A (hypoxia-inducible factor 1 alpha), and thus plays a role in the stimulation of hypoxia-induced genes such as VEGF. Defects in this gene are a cause of Rubinstein-Taybi syndrome and may also play a role in epithelial cancer. [provided by RefSeq, Jul 2008],
      • Function:
      • catalytic activity:Acetyl-CoA + histone = CoA + acetylhistone.,disease:Chromosomal aberrations involving EP300 may be a cause of acute myeloid leukemias. Translocation t(8;22)(p11;q13) with MYST3.,disease:Defects in EP300 are a cause of Rubinstein-Taybi syndrome (RSTS) [MIM:180849]. RSTS is an autosomal dominant disorder characterized by craniofacial abnormalities, broad thumbs, broad big toes, mental retardation and a propensity for development of malignancies.,disease:Defects in EP300 may play a role in epithelial cancer.,function:Functions as histone acetyltransferase and regulates transcription via chromatin remodeling. Acetylates all four core histones in nucleosomes. Histone acetylation gives an epigenetic tag for transcriptional activation. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein. Mediates cAMP-gene regulation by binding specifically
      • Subcellular Location:
      • histone acetyltransferase complex,nucleus,nucleoplasm,transcription factor complex,cytoplasm,
      • Expression:
      • Epithelium,Skin,
      • Products Images
      • Western Blot analysis of various cells using p300 Polyclonal Antibody
      • Western blot analysis of lysates from 293 cells, using p300 Antibody. The lane on the right is blocked with the synthesized peptide.