Nicalin Polyclonal Antibody

    • Catalog No.:YT3124
    • Applications:WB;IHC;IF;ELISA
    • Reactivity:Human;Mouse;Rat
      • Target:
      • Nicalin
      • Gene Name:
      • NCLN
      • Protein Name:
      • Nicalin
      • Human Swiss Prot No:
      • Q969V3
      • Mouse Swiss Prot No:
      • Q8VCM8
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human NCLN. AA range:474-523
      • Specificity:
      • Nicalin Polyclonal Antibody detects endogenous levels of Nicalin protein.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Polyclonal, Rabbit,IgG
      • Dilution:
      • WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000.. IF 1:50-200
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Other Name:
      • NCLN;Nicalin;Nicastrin-like protein
      • Observed Band(KD):
      • 63kD
      • Background:
      • function:May antagonize Nodal signaling and subsequent organization of axial structures during mesodermal patterning.,similarity:Belongs to the nicastrin family.,subunit:Interacts with NOMO2. Due to the strong similarity between NOMO1, NOMO2 and NOMO3, it probably also interacts with NOMO1 and NOMO3. Participates in a large protein complex, which is not related to the gamma-secretase complex.,tissue specificity:Highly expressed in pancreas and skeletal muscle and, at lower levels, in heart.,
      • Function:
      • function:May antagonize Nodal signaling and subsequent organization of axial structures during mesodermal patterning.,similarity:Belongs to the nicastrin family.,subunit:Interacts with NOMO2. Due to the strong similarity between NOMO1, NOMO2 and NOMO3, it probably also interacts with NOMO1 and NOMO3. Participates in a large protein complex, which is not related to the gamma-secretase complex.,tissue specificity:Highly expressed in pancreas and skeletal muscle and, at lower levels, in heart.,
      • Subcellular Location:
      • Endoplasmic reticulum membrane ; Single-pass membrane protein .
      • Expression:
      • Highly expressed in pancreas and skeletal muscle and, at lower levels, in heart.
      • Products Images
      • Immunohistochemical analysis of paraffin-embedded Human colon cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
      • Western blot analysis of lysates from HepG2 and HUVEC cells, using NCLN Antibody. The lane on the right is blocked with the synthesized peptide.