LATS1/2 Polyclonal Antibody

    • Catalog No.:YT2543
    • Applications:WB,IHC-p,IF(paraffin section),ELISA
    • Reactivity:Human,Mouse
      • Gene Name:
      • LATS1/LATS2
      • Protein Name:
      • Serine/threonine-protein kinase LATS1/2
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human LATS1/2. AA range:1041-1090
      • Specificity:
      • LATS1/2 Polyclonal Antibody detects endogenous levels of LATS1/2 protein.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Rabbit
      • Dilution:
      • IF(paraffin section) 1:50-200 Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -20°C/1 year
      • Other Name:
      • LATS1; WARTS; Serine/threonine-protein kinase LATS1; Large tumor suppressor homolog 1; WARTS protein kinase; h-warts; LATS2; KPM; Serine/threonine-protein kinase LATS2; Kinase phosphorylated during mitosis protein; Large tumor suppressor ho
      • MolecularWeight(Da):
      • 126870/120194
      • Observed Band(KD):
      • 130-140
      • Background:
      • large tumor suppressor kinase 1(LATS1) Homo sapiens The protein encoded by this gene is a putative serine/threonine kinase that localizes to the mitotic apparatus and complexes with cell cycle controller CDC2 kinase in early mitosis. The protein is phosphorylated in a cell-cycle dependent manner, with late prophase phosphorylation remaining through metaphase. The N-terminal region of the protein binds CDC2 to form a complex showing reduced H1 histone kinase activity, indicating a role as a negative regulator of CDC2/cyclin A. In addition, the C-terminal kinase domain binds to its own N-terminal region, suggesting potential negative regulation through interference with complex formation via intramolecular binding. Biochemical and genetic data suggest a role as a tumor suppressor. This is supported by studies in knockout mice showing development of soft-tissue sarcomas, ovarian stromal cell tumors and a high sensitivity to carcinogenic treatmen
      • Function:
      • catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,function:Tumor suppressor which plays a critical role in maintenance of ploidy through its actions in both mitotic progression and the G1 tetraploidy checkpoint. Negatively regulates G2/M transition by down-regulating CDC2 kinase activity. Involved in the control of p53 expression. Affects cytokinesis by regulating actin polymerization through negative modulation of LIMK1. May also play a role in endocrine function.,PTM:Autophosphorylated and phosphorylated during M-phase of the cell cycle. Phosphorylated by STK3 at Ser-909 and Thr-1079, which results in its activation. Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.,similarity:Contains 1 AGC-kinase C-terminal domain.,similarity:Contains 1 protein kinase domain.,
      • Subcellular Location:
      • spindle pole,intracellular,microtubule organizing center,cytosol,
      • Expression:
      • Brain,Endometrium,Epithelium,Fetal brain,Lung,
      • Products Images
      • Western Blot analysis of mouse-mscle cells using LATS1/2 Polyclonal Antibody diluted at 1:500
      • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using LATS1/2 Antibody. The picture on the right is blocked with the synthesized peptide.
      • Western blot analysis of LATS1/2 Antibody