JAK2 Polyclonal Antibody

    • Catalog No.:YT2426
    • Applications:IF;WB;IHC;ELISA
    • Reactivity:Human;Mouse;Rat
      • Target:
      • JAK2
      • Fields:
      • >>EGFR tyrosine kinase inhibitor resistance;>>Chemokine signaling pathway;>>PI3K-Akt signaling pathway;>>Necroptosis;>>Signaling pathways regulating pluripotency of stem cells;>>JAK-STAT signaling pathway;>>Th1 and Th2 cell differentiation;>>Th17 cell differentiation;>>Cholinergic synapse;>>Prolactin signaling pathway;>>Adipocytokine signaling pathway;>>AGE-RAGE signaling pathway in diabetic complications;>>Growth hormone synthesis, secretion and action;>>Leishmaniasis;>>Toxoplasmosis;>>Tuberculosis;>>Hepatitis B;>>Influenza A;>>Kaposi sarcoma-associated herpesvirus infection;>>Herpes simplex virus 1 infection;>>Pathways in cancer;>>Chemical carcinogenesis - receptor activation;>>PD-L1 expression and PD-1 checkpoint pathway in cancer;>>Lipid and atherosclerosis
      • Gene Name:
      • JAK2
      • Protein Name:
      • Tyrosine-protein kinase JAK2
      • Human Gene Id:
      • 3717
      • Human Swiss Prot No:
      • O60674
      • Mouse Swiss Prot No:
      • Q62120
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human JAK2. AA range:981-1030
      • Specificity:
      • JAK2 Polyclonal Antibody detects endogenous levels of JAK2 protein.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Polyclonal, Rabbit,IgG
      • Dilution:
      • IF 1:50-200 WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000. Not yet tested in other applications.
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Other Name:
      • JAK2;Tyrosine-protein kinase JAK2;Janus kinase 2;JAK-2
      • Observed Band(KD):
      • 130kD
      • Background:
      • This gene product is a protein tyrosine kinase involved in a specific subset of cytokine receptor signaling pathways. It has been found to be constituitively associated with the prolactin receptor and is required for responses to gamma interferon. Mice that do not express an active protein for this gene exhibit embryonic lethality associated with the absence of definitive erythropoiesis. [provided by RefSeq, Jul 2008],
      • Function:
      • catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,disease:Chromosomal aberrations involving JAK2 are found in both chronic and acute forms of eosinophilic, lymphoblastic and myeloid leukemia. Translocation t(8;9)(p22;p24) with PCM1 links the protein kinase domain of JAK2 to the major portion of PCM1. Translocation t(9;12)(p24;p13) with ETV6.,disease:Defects in JAK2 are a cause of acute myelogenous leukemia (AML) [MIM:601626]. AML is a malignant disease in which hematopoietic precursors are arrested in an early stage of development.,disease:Defects in JAK2 are a cause of susceptibility to Budd-Chiari syndrome [MIM:600880]. Budd-Chiari syndrome is a spectrum of disease states, including anatomic abnormalities and hypercoagulable disorders, resulting in hepatic venous outflow occlusion. Clinical manifestations observed in the majority of patients incl
      • Subcellular Location:
      • Endomembrane system ; Peripheral membrane protein . Cytoplasm . Nucleus .
      • Expression:
      • Ubiquitously expressed throughout most tissues.

      Shenlian extract attenuates myocardial ischaemia-reperfusion injury via inhibiting M1 macrophage polarization by silencing miR-155

      Shaoyao-Gancao Decoction Promoted Microglia M2 Polarization via the IL-13-Mediated JAK2/STAT6 Pathway to Alleviate Cerebral Ischemia-Reperfusion Injury WB Rat 1:400 penumbra tissue/

      Network pharmacology analysis and experimental validation to explore the mechanism of Shenlian extract on myocardial ischemia J Ethnopharmacol. 2022 Apr;288:114973. WB Rat 1:1000 Heart

      Glycerophospholipid metabolism is involved in rheumatoid arthritis pathogenesis by regulating the IL-6/JAK signaling pathway Biochem Bioph Res Co. 2022 Feb;:. WB Human 1:1000

      Glycogen metabolism regulates macrophage-mediated acute inflammatory responses. Nature Communications Nat Commun. 2020 Apr;11(1):1-16 WB Mouse 1:1000 BMDMs

      HIF‐1α facilitates osteocyte‐mediated osteoclastogenesis by activating JAK2/STAT3 pathway in vitro. JOURNAL OF CELLULAR PHYSIOLOGY 2019 Apr 29 WB Mouse 1:500 MLO-Y4 cell

      Carbon Ion Therapy Inhibits Esophageal Squamous Cell Carcinoma Metastasis by Upregulating STAT3 Through the JAK2/STAT3 Signaling Pathway. Frontiers in Public Health Front Public Health. 2020 Nov;0:801 WB Human 1:1000 ECA109 cell, KYSE150 cell

      Mitochondrial dysfunction-mediated decline in angiogenic capacity of endothelial progenitor cells is associated with capillary rarefaction in patients with hypertension via downregulation of CXCR4/JAK2/SIRT5 signaling. EBioMedicine Ebiomedicine. 2019 Apr;42:64 WB Rat 1:1000 EPCs

      Mitochondrial dysfunction-mediated decline in angiogenic capacity of endothelial progenitor cells is associated with capillary rarefaction in patients with hypertension via downregulation of CXCR4/JAK2/SIRT5 signaling. EBioMedicine Ebiomedicine. 2019 Apr;42:64 WB Rat 1:1000 EPCs

      miRNA-331-3p Affects the Proliferation, Metastasis, and Invasion of Osteosarcoma through SOCS1/JAK2/STAT3 Journal of Oncology Bing Wu WB Mouse

      Shenlian extract improves atherosclerosis by relieving adventitial inflammation. JOURNAL OF ETHNOPHARMACOLOGY Xiaoxin Zhu WB Rat vascular adventitial fibroblasts (VAFs)

      • Products Images
      • Immunofluorescence analysis of rat-lung tissue. 1,JAK2 Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
      • Immunofluorescence analysis of rat-spleen tissue. 1,JAK2 Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
      • Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1,JAK2 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
      • Western Blot analysis of various cells using JAK2 Polyclonal Antibody diluted at 1:2000
      • Western Blot analysis of HT29 cells using JAK2 Polyclonal Antibody diluted at 1:2000
      • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using JAK2 Antibody. The picture on the right is blocked with the synthesized peptide.
      • Western blot analysis of lysates from HT-29 cells, using JAK2 Antibody. The lane on the right is blocked with the synthesized peptide.