IRF-4 Polyclonal Antibody

    • Catalog No.:YT2399
    • Applications:WB;IHC;IF;ELISA
    • Reactivity:Human;Mouse
      • Target:
      • IRF-4
      • Fields:
      • >>Th17 cell differentiation
      • Gene Name:
      • IRF4
      • Protein Name:
      • Interferon regulatory factor 4
      • Human Gene Id:
      • 3662
      • Human Swiss Prot No:
      • Q15306
      • Mouse Swiss Prot No:
      • Q64287
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human IRF4. AA range:281-330
      • Specificity:
      • IRF-4 Polyclonal Antibody detects endogenous levels of IRF-4 protein.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Polyclonal, Rabbit,IgG
      • Dilution:
      • WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Other Name:
      • IRF4;MUM1;Interferon regulatory factor 4;IRF-4;Lymphocyte-specific interferon regulatory factor;LSIRF;Multiple myeloma oncogene 1;NF-EM5
      • Observed Band(KD):
      • 45kD
      • Background:
      • The protein encoded by this gene belongs to the IRF (interferon regulatory factor) family of transcription factors, characterized by an unique tryptophan pentad repeat DNA-binding domain. The IRFs are important in the regulation of interferons in response to infection by virus, and in the regulation of interferon-inducible genes. This family member is lymphocyte specific and negatively regulates Toll-like-receptor (TLR) signaling that is central to the activation of innate and adaptive immune systems. A chromosomal translocation involving this gene and the IgH locus, t(6;14)(p25;q32), may be a cause of multiple myeloma. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Aug 2010],
      • Function:
      • disease:A chromosomal aberration involving IRF4 may be a cause of multiple myeloma [MIM:254500]. Translocation t(6;14)(p25;q32) with the IgH locus.,function:Transcriptional activator. Binds to the interferon-stimulated response element (ISRE) of the MHC class I promoter. Binds the immunoglobulin lambda light chain enhancer, together with PU.1. Probably plays a role in ISRE-targeted signal transduction mechanisms specific to lymphoid cells.,induction:Not induced by interferons.,similarity:Belongs to the IRF family.,similarity:Contains 1 tryptophan pentad repeat DNA-binding domain.,subunit:Interacts with SPIB and DEF6.,tissue specificity:Lymphoid cells.,
      • Subcellular Location:
      • Nucleus.
      • Expression:
      • Lymphoid cells.
      • Products Images
      • Western Blot analysis of various cells using IRF-4 Polyclonal Antibody
      • Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
      • Western blot analysis of lysates from HeLa and Jurkat cells, using IRF4 Antibody. The lane on the right is blocked with the synthesized peptide.
      • Western blot analysis of the lysates from HT-29 cells using IRF4 antibody.