HuB/D Polyclonal Antibody
- Catalog No.:YT2261
- Applications:WB;IHC;IF;ELISA
- Reactivity:Human;Mouse;Rat
- Target:
- HuB/D
- Gene Name:
- ELAVL2/ELAVL4
- Protein Name:
- ELAV-like protein 2/ELAV-like protein 4
- Human Gene Id:
- 1993/1996
- Human Swiss Prot No:
- Q12926/P26378
- Mouse Gene Id:
- 15569/15572
- Rat Gene Id:
- 432358
- Rat Swiss Prot No:
- Q8CH84/O09032
- Immunogen:
- The antiserum was produced against synthesized peptide derived from human ELAV2/4. AA range:61-110
- Specificity:
- HuB/D Polyclonal Antibody detects endogenous levels of HuB/D protein.
- Formulation:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source:
- Polyclonal, Rabbit,IgG
- Dilution:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:40000. Not yet tested in other applications.
- Purification:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Concentration:
- 1 mg/ml
- Storage Stability:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Other Name:
- ELAVL2;HUB;ELAV-like protein 2;ELAV-like neuronal protein 1;Hu-antigen B;HuB;Nervous system-specific RNA-binding protein Hel-N1;ELAVL4;HUD;PNEM;ELAV-like protein 4;Hu-antigen D;HuD;Paraneoplastic encephalomyelitis antigen HuD
- Observed Band(KD):
- 40kD
- Background:
- ELAV like RNA binding protein 2(ELAVL2) Homo sapiens The protein encoded by this gene is a neural-specific RNA-binding protein that is known to bind to several 3' UTRs, including its own and also that of FOS and ID. The encoded protein may recognize a GAAA motif in the RNA. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jan 2010],
- Function:
- function:Binds RNA. Seems to recognize a GAAA motif. Can bind to its own 3'-UTR, the FOS 3'-UTR and the ID 3'-UTR.,similarity:Belongs to the RRM elav family.,similarity:Contains 3 RRM (RNA recognition motif) domains.,tissue specificity:Brain; neural-specific.,
- Subcellular Location:
- nucleoplasm,
- Expression:
- Brain; neural-specific.
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- Antibody-FAQs
- Products Images
- Western Blot analysis of Hela cells using HuB/D Polyclonal Antibody diluted at 1:1000
- Immunofluorescence analysis of HepG2 cells, using ELAV2/4 Antibody. The picture on the right is blocked with the synthesized peptide.
- Western blot analysis of lysates from Jurkat and HUVEC cells, using ELAV2/4 Antibody. The lane on the right is blocked with the synthesized peptide.
- Western blot analysis of the lysates from 293 cells using ELAV2/4 antibody.
- Immunohistochemical analysis of paraffin-embedded human spleen. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).