G3BP1 Polyclonal Antibody

    • Catalog No.:YT1808
    • Applications:WB;IHC;IF;ELISA
    • Reactivity:Human;Mouse;Monkey
      • Target:
      • G3BP1
      • Gene Name:
      • G3BP1
      • Protein Name:
      • Ras GTPase-activating protein-binding protein 1
      • Human Swiss Prot No:
      • Q13283
      • Mouse Swiss Prot No:
      • P97855
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human G3BP-1. AA range:199-248
      • Specificity:
      • G3BP1 Polyclonal Antibody detects endogenous levels of G3BP1 protein.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Polyclonal, Rabbit,IgG
      • Dilution:
      • WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Other Name:
      • G3BP1;G3BP;Ras GTPase-activating protein-binding protein 1;G3BP-1;ATP-dependent DNA helicase VIII;hDH VIII;GAP SH3 domain-binding protein 1
      • Observed Band(KD):
      • 60kD
      • Background:
      • This gene encodes one of the DNA-unwinding enzymes which prefers partially unwound 3'-tailed substrates and can also unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion. This enzyme is a member of the heterogeneous nuclear RNA-binding proteins and is also an element of the Ras signal transduction pathway. It binds specifically to the Ras-GTPase-activating protein by associating with its SH3 domain. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Jul 2008],
      • Function:
      • cofactor:Magnesium. Required for helicase activity.,domain:The NTF2 domain mediates multimerization.,function:May be a regulated effector of stress granule assembly. Phosphorylation-dependent sequence-specific endoribonuclease in vitro. Cleaves exclusively between cytosine and adenine and cleaves MYC mRNA preferentially at the 3'-UTR. ATP- and magnesium-dependent helicase. Unwinds preferentially partial DNA and RNA duplexes having a 17 bp annealed portion and either a hanging 3' tail or hanging tails at both 5'- and 3'-ends. Unwinds DNA/DNA, RNA/DNA, and RNA/RNA substrates with comparable efficiency. Acts unidirectionally by moving in the 5' to 3' direction along the bound single-stranded DNA.,PTM:Arg-435 is dimethylated, probably to asymmetric dimethylarginine.,PTM:Phosphorylated exclusively on serine residues. Hyperphosphorylated in quiescent fibroblasts. Hypophosphorylation leads to a
      • Subcellular Location:
      • Cytoplasm, cytosol . Perikaryon . Cytoplasm, Stress granule . Nucleus . Cytoplasmic in proliferating cells (PubMed:11604510). Cytosolic and partially nuclear in resting cells (PubMed:11604510). Recruited to stress granules in response to arsenite treatment (PubMed:12642610, PubMed:20180778). The unphosphorylated form is recruited to stress granules (PubMed:12642610). HRAS signaling contributes to this process by regulating G3BP dephosphorylation (PubMed:12642610). .
      • Expression:
      • Ubiquitous.
      • Products Images
      • Western Blot analysis of various cells using G3BP1 Polyclonal Antibody
      • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using G3BP-1 Antibody. The picture on the right is blocked with the synthesized peptide.
      • Western blot analysis of lysates from HeLa cells, using G3BP-1 Antibody. The lane on the right is blocked with the synthesized peptide.