• Target：
• ERK 1/2

• Fields：
• >>EGFR tyrosine kinase inhibitor resistance;>>Endocrine resistance;>>Platinum drug resistance;>>MAPK signaling pathway;>>ErbB signaling pathway;>>Ras signaling pathway;>>Rap1 signaling pathway;>>cGMP-PKG signaling pathway;>>cAMP signaling pathway;>>Chemokine signaling pathway;>>HIF-1 signaling pathway;>>FoxO signaling pathway;>>Sphingolipid signaling pathway;>>Phospholipase D signaling pathway;>>Oocyte meiosis;>>Autophagy - animal;>>mTOR signaling pathway;>>PI3K-Akt signaling pathway;>>Apoptosis;>>Cellular senescence;>>Adrenergic signaling in cardiomyocytes;>>Vascular smooth muscle contraction;>>TGF-beta signaling pathway;>>Axon guidance;>>VEGF signaling pathway;>>Apelin signaling pathway;>>Osteoclast differentiation;>>Focal adhesion;>>Adherens junction;>>Gap junction;>>Signaling pathways regulating pluripotency of stem cells;>>Platelet activation;>>Neutrophil extracellular trap formation;>>Toll-like receptor signaling pathway;>>NOD-like receptor signaling pathway;>>C-type lectin recep

• Gene Name：
• MAPK1/MAPK3

• Protein Name：
• Mitogen-activated protein kinase 3

• Human Gene Id：
• 5595

• Human Swiss Prot No：
• P27361/P28482

• Mouse Gene Id：
• 26417/26413

• Rat Gene Id：
• 50689/116590

• Rat Swiss Prot No：
• P21708/P63086

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human p44/42 MAPK. AA range:330-379

• Specificity：
• ERK 1/2 Polyclonal Antibody detects endogenous levels of ERK 1/2 protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• IF 1:50-200 WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000. Not yet tested in other applications.

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• MAPK1;ERK2;PRKM1;PRKM2;Mitogen-activated protein kinase 1;MAP kinase 1;MAPK 1;ERT1;Extracellular signal-regulated kinase 2;ERK-2;MAP kinase isoform p42;p42-MAPK;Mitogen-activated protein kinase 2;MAP kinase 2;MAPK 2;MAPK3;MAPK3;ERK1;PRKM3;Mitogen-activated protein kinase 3;MAP kinase 3;MAPK 3;ERT2;Extracellular signal-regulated kinase 1;ERK-1;Insulin-stimulated MAP2 kinase;MAP kinase isoform p44;p44-MAPK;Microtubule-associated protein 2 kinase;p

• Observed Band(KD)：
• 42,44kD

• Background：
• The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described. [provided by RefSeq, Jul 2008],

• Function：
• catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,domain:The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.,enzyme regulation:Activated by tyrosine phosphorylation in response to insulin and NGF.,function:Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4).,PTM:Dually phosphorylated on Thr-202 and Tyr-204, which activates the enzyme.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinas

• Subcellular Location：
• Cytoplasm . Nucleus. Membrane, caveola . Cell junction, focal adhesion . Autophosphorylation at Thr-207 promotes nuclear localization (PubMed:19060905). PEA15-binding redirects the biological outcome of MAPK3 kinase-signaling by sequestering MAPK3 into the cytoplasm (By similarity). .

• Expression：
• Epithelium,Eye,Hepatoma,Human cervix,Lymph,

High expression level of CXCL1/GROα is linked to advanced stage and worse survival in uterine cervical cancer and facilitates tumor cell malignant processes WB Human 1:1000 /HeLa cell

Circular RNA circNRIP1 promotes migration and invasion in cervical cancer by sponging miR-629-3p and regulating the PTP4A1/ERK1/2 pathway. Cell Death & Disease Cell Death Dis. 2020 May;11(5):1-15 WB Human HeLa cell,SiHa cell

Telekin suppresses human hepatocellular carcinoma cells in vitro by inducing G 2 /M phase arrest via the p38 MAPK signaling pathway. ACTA PHARMACOLOGICA SINICA Acta Pharmacol Sin. 2014 Sep;35(10):1311-1322 WB Human HepG2 cell

LASP2 is downregulated in human liver cancer and contributes to hepatoblastoma cell malignant phenotypes through MAPK/ERK pathway. BIOMEDICINE & PHARMACOTHERAPY Biomed Pharmacother. 2020 Jul;127:110154 WB Human 1:1000 HepG2 cell

Effects of hydroxysafflor yellow A on rats with collagen-induced arthritis. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS Biochem Bioph Res Co. 2021 Sep;570:26 WB Rat 1:500 Synovial

Crassifoside H improve the depressive-like behavior of rats under chronic unpredictable mild stress: Possible involved mechanisms. BRAIN RESEARCH BULLETIN Brain Res Bull. 2017 Oct;135:77 WB Rat 1:1000 hippocampus , hypothalamus

Preventative effects of resveratrol and estradiol on streptozotocin-induced diabetes in ovariectomized mice and the related mechanisms. PLoS One 2018 Oct 01 WB Mouse Skeletal muscle,myocardial tissues

CXCL5, the upregulated chemokine in patients with uterine cervix cancer, in vivo and in vitro contributes to oncogenic potential of Hela uterine cervix cancer cells. BIOMEDICINE & PHARMACOTHERAPY Biomed Pharmacother. 2018 Nov;107:1496 WB Mouse,Human 1:1000 Hela cell-xenografts Hela cell

Novel paeonol derivatives: Design, synthesis and anti-inflammatory activity in vitro and in vivo. BIOORGANIC CHEMISTRY Bioorg Chem. 2020 May;98:103735 ChIP Mouse RAW264.7 cell

The mechanism of mulberry leaves against renal tubular interstitial fibrosis through ERK1/2 signaling pathway was predicted by network pharmacology and validated in human tubular epithelial cells. PHYTOTHERAPY RESEARCH 2019 Jun 17 WB Human 1:1000 HK-2 cell

Ad-p53 enhances the sensitivity of triple-negative breast cancer MDA-MB-468 cells to the EGFR inhibitor gefitinib. ONCOLOGY REPORTS 2014 Dec 11 WB Human MDA-MB-468 cell

Matrine inhibits BCR/ABL mediated ERK/MAPK pathway in human leukemia cells. Oncotarget Oncotarget. 2017 Dec 12; 8(65): 108880–108889 WB Human 1:500 ,1:2000 K562 cell, HL-60 cell

The MEK/ERK/CREB signaling pathway is involved in atrazine induced hippocampal neurotoxicity in Sprague Dawley rats. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY 2018 Dec 20 WB Rat 1:1500 hippocampal tissue

Mesenchymal stem cell‑derived extracellular vesicles promote the in vitro proliferation and migration of breast cancer cells through the activation of the ERK pathway. INTERNATIONAL JOURNAL OF ONCOLOGY Int J Oncol. 2019 May;54(5):1843-1852 WB Human 1:1000 hUC-MSC-EVs

Negative Regulation of Grb10 Interacting GYF Protein 2 on Insulin-Like Growth Factor-1 Receptor Signaling Pathway Caused Diabetic Mice Cognitive Impairment. PLoS One 2014 Sep 30 WB Mouse 1∶1000 hippocampus

Astragalus Polysaccharide Eases G1 Phase-Correlative Bystander Effects through Mediation of TGF-βR/MAPK/ROS Signal Pathway After Carbon Ion Irradiation in BMSCs. AMERICAN JOURNAL OF CHINESE MEDICINE Am J Chinese Med. 2019 May;47(03):595-612 WB Human BMSCs

Serum proteomics analysis based on label-free revealed the protective effect of Chinese herbal formula Gu-Ben-Fang-Xiao. BIOMEDICINE & PHARMACOTHERAPY Biomed Pharmacother. 2019 Nov;119:109390 WB Mouse 1:1000 lung tissue

CXCL3 overexpression promotes the tumorigenic potential of uterine cervical cancer cells via the MAPK/ERK pathway. JOURNAL OF CELLULAR PHYSIOLOGY J Cell Physiol. 2020 May;235(5):4756-4765 WB Human 1:1000 HeLa cell

SCF/C-KIT signaling modulates tryptase expression in acute myeloid leukemia cells. INTERNATIONAL JOURNAL OF HEMATOLOGY Int J Hematol. 2014 Jun;99(6):750-757 WB Human U937 cell

Protective effects of flavonoids from the leaves of Carya cathayensis Sarg. against H₂O₂‑induced oxidative damage and apoptosis in vitro. Experimental and Therapeutic Medicine Exp Ther Med. 2021 Dec;22(6):1-11 WB Rat 1:1000 Rat aortic endothelial cells (RAECs)

CTGF enhances resistance to 5-FU-mediating cell apoptosis through FAK/MEK/ERK signal pathway in colorectal cancer. OncoTargets and Therapy Oncotargets Ther. 2016; 9: 7285–7295 WB Human 1:200 HT-29 cell, SW620 cell

Specific matrix metalloproteinases and calcification factors are associated with the vulnerability of human carotid plaque. Experimental and Therapeutic Medicine 2018 Sep 01 WB Human 1:1000 Carotid plaques

Aβ40 Promotes the Osteoblastic Differentiation of Aortic Valve Interstitial Cells through the RAGE Pathway. Current Medical Science 2020 Oct 29 WB Human 1:1000 Acute aortic aortic valve interstitial cells (AVICs)

Man, XiaXia, et al. "High-level expression of CXCL1/GROα in uterine cervix cancer is linked to advanced stage and facilitates tumor cell malignant processes in an autocrine/paracrine manner." (2020).

Qingjie, K. A. N. G., and X. I. A. N. G. Zheng. "Effect of glioma-associated oncogene homolog 2 silencing on proliferation and apoptosis of colon cancer cell line SW620." China Oncology 30.10 (2020): 785-790.

Meng, Xiangli, et al. "Integrin beta 4 (ITGB4) and its tyrosine-1510 phosphorylation promote pancreatic tumorigenesis and regulate the MEK1-ERK1/2 signaling pathway." Bosnian journal of basic medical sciences 20.1 (2020): 106.

康清杰, and 向征. "沉默胶质瘤相关癌基因同源物 2 基因对结肠癌细胞系 SW620 增殖和凋亡的影响." 中国癌症杂志 30.10 (2020): 785-790.

SCF/C-KIT signaling modulates tryptase expression in acute myeloid leukemia cells. INTERNATIONAL JOURNAL OF HEMATOLOGY Int J Hematol. 2014 Jun;99(6):750-757 WB Human U937 cell

Specific matrix metalloproteinases and calcification factors are associated with the vulnerability of human carotid plaque. Experimental and Therapeutic Medicine 2018 Sep 01 WB Human 1:1000 Carotid plaques

Mesenchymal stem cell-derived extracellular vesicles promote apoptosis in RSC96 Schwann cells through the activation of the ERK pathway. International Journal of Clinical and Experimental Pathology Int J Clin Exp Patho. 2018; 11(11): 5157–5170 WB Rat RSC96 cell

Ershiwuwei Lvxue Pill alleviates rheumatoid arthritis by different pathways and produces changes in the gut microbiota PHYTOMEDICINE Shaohui Wang WB Rat

Nanocarrier of Pin1 inhibitor based on supercritical fluid technology inhibits cancer metastasis by blocking multiple signaling pathways Regenerative Biomaterials Dayun Yang WB Human HuH7 cell,HepG2 cell

HMGB1 released from dead tumor cells after insufficient radiofrequency ablation promotes progression of HCC residual tumor via ERK1/2 pathway INTERNATIONAL JOURNAL OF HYPERTHERMIA Baoming Luo WB Human HepG2 cell,Huh7 cell

Silencing LCN2 suppresses oral squamous cell carcinoma progression by reducing EGFR signal activation and recycling JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH Zhiquan Huang WB Human CAL-27ER cell,HN-6ER cell,CAL-27 cell,HN-6 cell

FGF12 regulates cell cycle gene expression and promotes follicular granulosa cell proliferation through ERK phosphorylation in geese. Xingyong Chen WB Geese 1:1000 granulosa cell

Dietary antioxidant quercetin overcomes the acquired resistance of Sorafenib in Sorafenib-resistant hepatocellular carcinoma cells through epidermal growth factor receptor signaling inactivation. Zhou Fuqiong WB Mouse，Human 1:1000 Huh7 cell-xenograft Huh7 cell

mGluR1/IP3/ERK signaling pathway regulates vestibular compensation in ON UBCs of the cerebellar flocculus. CNS Neuroscience & Therapeutics Sulin Zhang WB Rat 1:1000 Flocculus tissue

Exploring the role and mechanism of Astragalus membranaceus and radix paeoniae rubra in idiopathic pulmonary fibrosis through network pharmacology and experimental validation. Scientific Reports Quanyu Du IHC Rat lung tissue

Design and synthesis of pterostilbene derivatives bearing triazole moiety that might treat DSS-induced colitis in mice through modulation of NF-κB/MAPK signaling pathways EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY Liuzeng Chen WB Mouse 1:1000 RAW264.7 cell

Mesenchymal stem cells alleviate sepsis-induced acute lung injury by blocking neutrophil extracellular traps formation and inhibiting ferroptosis in rats PeerJ TieNan Wang WB Rat 1:2000 lung tissue mesenchymal stem cells (MSCs)

Design, synthesis, in vitro and in vivo biological evaluation of pterostilbene derivatives for anti-inflammation therapy JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY Liuzeng Chen WB Mouse 1:1000 RAW264.7 cell

The gut microbiota contributes to the infection of bovine viral diarrhea virus in mice JOURNAL OF VIROLOGY Zecai Zhang WB Mouse peripheral blood lymphocytes (PBL)

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