Csk Polyclonal Antibody

    • Catalog No.:YT1131
    • Applications:WB;IHC;IF;ELISA
    • Reactivity:Human;Mouse;Rat
      • Target:
      • Csk
      • Fields:
      • >>Epithelial cell signaling in Helicobacter pylori infection
      • Gene Name:
      • CSK
      • Protein Name:
      • Tyrosine-protein kinase CSK
      • Human Gene Id:
      • 1445
      • Human Swiss Prot No:
      • P41240
      • Mouse Swiss Prot No:
      • P41241
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human CSK. AA range:330-379
      • Specificity:
      • Csk Polyclonal Antibody detects endogenous levels of Csk protein.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Polyclonal, Rabbit,IgG
      • Dilution:
      • WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Other Name:
      • CSK;Tyrosine-protein kinase CSK;C-Src kinase;Protein-tyrosine kinase CYL
      • Observed Band(KD):
      • 50kD
      • Background:
      • catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,function:Specifically phosphorylates 'Tyr-504' on LCK, which acts as a negative regulatory site. Can also act on the LYN and FYN kinases.,PTM:Autophosphorylation of Tyr-304 occurs only at abnormally high CSK concentrations in vitro.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family. CSK subfamily.,similarity:Contains 1 protein kinase domain.,similarity:Contains 1 SH2 domain.,similarity:Contains 1 SH3 domain.,subcellular location:Mainly cytoplasmic, also present in lipid rafts.,subunit:Interacts with PTPN8 (By similarity). Interacts with phosphorylated SIT1, PAG1, LIME1 and TGFB1I1.,tissue specificity:Expressed in lung and macrophages.,
      • Function:
      • catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,function:Specifically phosphorylates 'Tyr-504' on LCK, which acts as a negative regulatory site. Can also act on the LYN and FYN kinases.,PTM:Autophosphorylation of Tyr-304 occurs only at abnormally high CSK concentrations in vitro.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family. CSK subfamily.,similarity:Contains 1 protein kinase domain.,similarity:Contains 1 SH2 domain.,similarity:Contains 1 SH3 domain.,subcellular location:Mainly cytoplasmic, also present in lipid rafts.,subunit:Interacts with PTPN8 (By similarity). Interacts with phosphorylated SIT1, PAG1, LIME1 and TGFB1I1.,tissue specificity:Expressed in lung and macrophages.,
      • Subcellular Location:
      • Cytoplasm . Cell membrane . Mainly cytoplasmic, also present in lipid rafts. .
      • Expression:
      • Expressed in lung and macrophages.
      • Products Images
      • Western Blot analysis of various cells using Csk Polyclonal Antibody diluted at 1:1000
      • Western Blot analysis of 22RV1 cells using Csk Polyclonal Antibody diluted at 1:1000
      • Immunohistochemistry analysis of paraffin-embedded human brain tissue, using CSK Antibody. The picture on the right is blocked with the synthesized peptide.
      • Western blot analysis of lysates from Raw264.7 cells, treated with UV 5', using CSK Antibody. The lane on the right is blocked with the synthesized peptide.