CD34 Polyclonal Antibody

    • Catalog No.:YT0757
    • Applications:WB,IHC-p,IF(paraffin section),ELISA
    • Reactivity:Human,Mouse
      • Gene Name:
      • CD34
      • Protein Name:
      • Hematopoietic progenitor cell antigen CD34
      • Human Gene Id:
      • 947
      • Human Swiss Prot No:
      • P28906
      • Mouse Swiss Prot No:
      • Q64314
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human CD34. AA range:208-257
      • Specificity:
      • CD34 Polyclonal Antibody detects endogenous levels of CD34 protein.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Rabbit
      • Dilution:
      • IF(paraffin section) 1:50-200 IHC-p: 100-300.Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -20°C/1 year
      • Other Name:
      • CD34; Hematopoietic progenitor cell antigen CD34; CD antigen CD34
      • MolecularWeight(Da):
      • 40716
      • Observed Band(KD):
      • 36
      • Background:
      • CD34 molecule(CD34) Homo sapiens The protein encoded by this gene may play a role in the attachment of stem cells to the bone marrow extracellular matrix or to stromal cells. This single-pass membrane protein is highly glycosylated and phosphorylated by protein kinase C. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011],
      • Function:
      • alternative products:Both isoforms are expressed on the cell surface. CD34-T/CD34-F ratio increases with cell differentiation,developmental stage:On early hematopoietic progenitor cells.,disease:Abnormal CD34 expression in leukemogenesis.,function:Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.,online information:CD34 entry,PTM:Highly glycosylated.,PTM:Phosphorylated on serine residues by PKC.,similarity:Belongs to the CD34 family.,tissue specificity:Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tiss
      • Subcellular Location:
      • cytoplasm,lysosome,plasma membrane,integral component of plasma membrane,external side of plasma membrane,basal plasma membrane,integral component of membrane,apical plasma membrane,glomerular endothelium fenestra,intercellular bridge,perinuclear
      • Expression:
      • Bone marrow,Heart,Megakaryoblast,Placenta,Rectum tumor,Skin,
      • Products Images
      • Western Blot analysis of various cells using CD34 Polyclonal Antibody diluted at 1:2000
      • Western Blot analysis of HepG2 cells using CD34 Polyclonal Antibody diluted at 1:2000
      • The picture was kindly provided by our customer
      • Immunohistochemical analysis of paraffin-embedded Human colon. 1, Antibody was diluted at 1:200(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
      • Immunohistochemical analysis of paraffin-embedded Human colon. 1, Antibody was diluted at 1:200(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
      • Immunohistochemical analysis of paraffin-embedded Human colon. 1, Antibody was diluted at 1:200(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
      • Immunohistochemical analysis of paraffin-embedded Human kidney. 1, Antibody was diluted at 1:200(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
      • Immunohistochemical analysis of paraffin-embedded Human kidney. 1, Antibody was diluted at 1:200(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
      • Immunohistochemical analysis of paraffin-embedded Human kidney. 1, Antibody was diluted at 1:200(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
      • Western blot analysis of lysate from HepG2 cells, using CD34 antibody.