Caspase-7 Polyclonal Antibody

    • Catalog No.:YT0659
    • Applications:WB,IHC-p,ELISA
    • Reactivity:Human
    • Description
    • Gene Name:
    • CASP7
    • Protein Name:
    • Caspase-7
    • Human Gene Id:
    • 840
    • Human Swiss Prot No:
    • P55210
    • Mouse Swiss Prot No:
    • P97864
    • Immunogen:
    • The antiserum was produced against synthesized peptide derived from human Caspase-7. AA range:45-94
    • Specificity:
    • Caspase-7 Polyclonal Antibody detects endogenous levels of Caspase-7 protein.
    • Formulation:
    • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
    • Source:
    • Rabbit
    • Dilution:
    • Western Blot: 1/500 - 1/2000.IHC-p:1:50-300 ELISA: 1/20000. Not yet tested in other applications.
    • Purification:
    • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
    • Concentration:
    • 1 mg/ml
    • Storage Stability:
    • -20°C/1 year
    • Other Name:
    • CASP7; MCH3; Caspase-7; CASP-7; Apoptotic protease Mch-3; CMH-1; ICE-like apoptotic protease 3; ICE-LAP3
    • MolecularWeight(Da):
    • 34277
    • Observed Band(KD):
    • 35
    • Background:
    • CASP7 encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. The precursor of the encoded protein (caspase 7) is cleaved by caspase 3 and 10, is activated upon cell death stimuli and induces apoptosis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for CASP7.
    • Function:
    • release of cytochrome c from mitochondria, proteolysis, apoptosis, mitochondrion organization, heart development,cell death, apoptotic mitochondrial changes, response to radiation, response to UV, response to light stimulus,response to abiotic stimulus, programmed cell death, death,
    • Products Images
    • Western Blot analysis of various cells using Caspase-7 Polyclonal Antibody diluted at 1:1000
    • Immunohistochemical analysis of paraffin-embedded Human stomach. 1, Antibody was diluted at 1:400(4°,overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
    • Immunohistochemical analysis of paraffin-embedded Human stomach. 1, Antibody was diluted at 1:400(4°,overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
    • Immunohistochemical analysis of paraffin-embedded Human stomach. 1, Antibody was diluted at 1:400(4°,overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
    • Immunohistochemistry analysis of Caspase-7 antibody in paraffin-embedded human breast carcinoma tissue.
    • Western blot analysis of lysate from HT-29cells, using Caspase-7 antibody.