Caspase-7 Polyclonal Antibody

    • Catalog No.:YT0659
    • Applications:WB,IHC-p,ELISA
    • Reactivity:Human
      • Gene Name:
      • CASP7
      • Protein Name:
      • Caspase-7
      • Human Gene Id:
      • 840
      • Human Swiss Prot No:
      • P55210
      • Mouse Swiss Prot No:
      • P97864
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human Caspase-7. AA range:45-94
      • Specificity:
      • Caspase-7 Polyclonal Antibody detects endogenous levels of Caspase-7 protein.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Rabbit
      • Dilution:
      • Western Blot: 1/500 - 1/2000.IHC-p:1:50-300 ELISA: 1/20000. Not yet tested in other applications.
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -20°C/1 year
      • Other Name:
      • CASP7; MCH3; Caspase-7; CASP-7; Apoptotic protease Mch-3; CMH-1; ICE-like apoptotic protease 3; ICE-LAP3
      • MolecularWeight(Da):
      • 34277
      • Observed Band(KD):
      • 35
      • Background:
      • caspase 7(CASP7) Homo sapiens This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. The precursor of the encoded protein is cleaved by caspase 3 and 10, is activated upon cell death stimuli and induces apoptosis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012],
      • Function:
      • catalytic activity:Strict requirement for an Asp residue at position P1 and has a preferred cleavage sequence of Asp-Glu-Val-Asp-|-.,enzyme regulation:Inhibited by isatin sulfonamides.,function:Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Overexpression promotes programmed cell death.,PTM:Cleavages by granzyme B or caspase-10 generate the two active subunits. Propeptide domains can also be cleaved efficiently by caspase-3. Active heterodimers between the small subunit of caspase-7 and the large subunit of caspase-3, and vice versa, also occur.,similarity:Belongs to the peptidase C14A family.,subunit:Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed
      • Subcellular Location:
      • nucleus,nucleoplasm,cytoplasm,cytosol,intracellular membrane-bounded organelle,
      • Expression:
      • Fetal lung,Fetal spleen,Skin,Spleen,T-cell,
      • Products Images
      • Western Blot analysis of various cells using Caspase-7 Polyclonal Antibody diluted at 1:1000
      • Immunohistochemical analysis of paraffin-embedded Human stomach. 1, Antibody was diluted at 1:400(4°,overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
      • Immunohistochemical analysis of paraffin-embedded Human stomach. 1, Antibody was diluted at 1:400(4°,overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
      • Immunohistochemical analysis of paraffin-embedded Human stomach. 1, Antibody was diluted at 1:400(4°,overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
      • Immunohistochemistry analysis of Caspase-7 antibody in paraffin-embedded human breast carcinoma tissue.
      • Western blot analysis of lysate from HT-29cells, using Caspase-7 antibody.