CARD 11 Polyclonal Antibody

    • Catalog No.:YT0635
    • Applications:WB;IHC;IF;ELISA
    • Reactivity:Human;Mouse
      • Target:
      • CARD11
      • Fields:
      • >>NF-kappa B signaling pathway;>>T cell receptor signaling pathway;>>B cell receptor signaling pathway
      • Gene Name:
      • CARD11
      • Protein Name:
      • Caspase recruitment domain-containing protein 11
      • Human Swiss Prot No:
      • Q9BXL7
      • Mouse Swiss Prot No:
      • Q8CIS0
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human CARD11. AA range:10-59
      • Specificity:
      • CARD 11 Polyclonal Antibody detects endogenous levels of CARD 11 protein.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Polyclonal, Rabbit,IgG
      • Dilution:
      • WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:40000. Not yet tested in other applications.
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Other Name:
      • CARD11;CARMA1;Caspase recruitment domain-containing protein 11;CARD-containing MAGUK protein 1;Carma 1
      • Observed Band(KD):
      • 130kD
      • Background:
      • The protein encoded by this gene belongs to the membrane-associated guanylate kinase (MAGUK) family, a class of proteins that functions as molecular scaffolds for the assembly of multiprotein complexes at specialized regions of the plasma membrane. This protein is also a member of the CARD protein family, which is defined by carrying a characteristic caspase-associated recruitment domain (CARD). This protein has a domain structure similar to that of CARD14 protein. The CARD domains of both proteins have been shown to specifically interact with BCL10, a protein known to function as a positive regulator of cell apoptosis and NF-kappaB activation. When expressed in cells, this protein activated NF-kappaB and induced the phosphorylation of BCL10. [provided by RefSeq, Jul 2008],
      • Function:
      • caution:Supposed to contain a SH3 domain which is not detected by PROSITE, Pfam or SMART.,function:Activates NF-kappa-B via BCL10 and IKK. Stimulates the phosphorylation of BCL10.,similarity:Contains 1 CARD domain.,similarity:Contains 1 guanylate kinase-like domain.,similarity:Contains 1 PDZ (DHR) domain.,subunit:CARD11 and BCL10 bind to each other by CARD-CARD interaction.,tissue specificity:Detected in adult peripheral blood leukocytes, thymus, spleen and liver. Also found in promyelocytic leukemia HL-60 cells, chronic myelogenous leukemia K562 cells, Burkitt's lymphoma Raji cells and colorectal adenocarcinoma SW480 cells. Not detected in HeLa S3, Molt-4, A549 and G431 cells.,
      • Subcellular Location:
      • Cytoplasm . Membrane raft . Colocalized with DPP4 in membrane rafts. .
      • Expression:
      • Detected in adult peripheral blood leukocytes, thymus, spleen and liver. Also found in promyelocytic leukemia HL-60 cells, chronic myelogenous leukemia K-562 cells, Burkitt's lymphoma Raji cells and colorectal adenocarcinoma SW480 cells. Not detected in HeLaS3, MOLT-4, A-549 and G431 cells.
      • Products Images
      • Western Blot analysis of various cells using CARD 11 Polyclonal Antibody
      • Western blot analysis of lysates from COLO205 cells, using CARD11 Antibody. The lane on the right is blocked with the synthesized peptide.
      • Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Tris-EDTA,pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200(4° overnight.3,Secondary antibody was diluted at 1:200(room temperature, 45min).