CARD 11 Polyclonal Antibody

    • Catalog No.:YT0635
    • Applications:WB,IHC-p,IF,ELISA
    • Reactivity:Human,Mouse
      • Gene Name:
      • CARD11
      • Protein Name:
      • Caspase recruitment domain-containing protein 11
      • Human Swiss Prot No:
      • Q9BXL7
      • Mouse Swiss Prot No:
      • Q8CIS0
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human CARD11. AA range:10-59
      • Specificity:
      • CARD 11 Polyclonal Antibody detects endogenous levels of CARD 11 protein.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Rabbit
      • Dilution:
      • Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -20°C/1 year
      • Other Name:
      • CARD11; CARMA1; Caspase recruitment domain-containing protein 11; CARD-containing MAGUK protein 1; Carma 1
      • MolecularWeight(Da):
      • 132658
      • Observed Band(KD):
      • 130
      • Background:
      • caspase recruitment domain family member 11(CARD11) Homo sapiens The protein encoded by this gene belongs to the membrane-associated guanylate kinase (MAGUK) family, a class of proteins that functions as molecular scaffolds for the assembly of multiprotein complexes at specialized regions of the plasma membrane. This protein is also a member of the CARD protein family, which is defined by carrying a characteristic caspase-associated recruitment domain (CARD). This protein has a domain structure similar to that of CARD14 protein. The CARD domains of both proteins have been shown to specifically interact with BCL10, a protein known to function as a positive regulator of cell apoptosis and NF-kappaB activation. When expressed in cells, this protein activated NF-kappaB and induced the phosphorylation of BCL10. [provided by RefSeq, Jul 2008],
      • Function:
      • caution:Supposed to contain a SH3 domain which is not detected by PROSITE, Pfam or SMART.,function:Activates NF-kappa-B via BCL10 and IKK. Stimulates the phosphorylation of BCL10.,similarity:Contains 1 CARD domain.,similarity:Contains 1 guanylate kinase-like domain.,similarity:Contains 1 PDZ (DHR) domain.,subunit:CARD11 and BCL10 bind to each other by CARD-CARD interaction.,tissue specificity:Detected in adult peripheral blood leukocytes, thymus, spleen and liver. Also found in promyelocytic leukemia HL-60 cells, chronic myelogenous leukemia K562 cells, Burkitt's lymphoma Raji cells and colorectal adenocarcinoma SW480 cells. Not detected in HeLa S3, Molt-4, A549 and G431 cells.,
      • Subcellular Location:
      • immunological synapse,cytoplasm,cytosol,plasma membrane,T cell receptor complex,membrane raft,extracellular exosome,
      • Expression:
      • Spleen,
      • Products Images
      • Western Blot analysis of various cells using CARD 11 Polyclonal Antibody
      • Western blot analysis of lysates from COLO205 cells, using CARD11 Antibody. The lane on the right is blocked with the synthesized peptide.