Arrestin-β-1 Polyclonal Antibody

    • Catalog No.:YT0344
    • Applications:WB;IHC;IF;ELISA
    • Reactivity:Human;Mouse;Rat
      • Target:
      • Arrestin 1
      • Fields:
      • >>MAPK signaling pathway;>>Chemokine signaling pathway;>>Endocytosis;>>Hedgehog signaling pathway;>>Dopaminergic synapse;>>Olfactory transduction;>>Relaxin signaling pathway;>>Parathyroid hormone synthesis, secretion and action;>>GnRH secretion;>>Morphine addiction;>>Chemical carcinogenesis - receptor activation
      • Gene Name:
      • ARRB1
      • Protein Name:
      • Beta-arrestin-1
      • Human Gene Id:
      • 408
      • Human Swiss Prot No:
      • P49407
      • Mouse Swiss Prot No:
      • Q8BWG8
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human ARRB1. AA range:369-418
      • Specificity:
      • Arrestin-β-1 Polyclonal Antibody detects endogenous levels of Arrestin-β-1 protein.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Polyclonal, Rabbit,IgG
      • Dilution:
      • WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Other Name:
      • ARRB1;ARR1;Beta-arrestin-1;Arrestin beta-1
      • Observed Band(KD):
      • 50kD
      • Background:
      • Members of arrestin/beta-arrestin protein family are thought to participate in agonist-mediated desensitization of G-protein-coupled receptors and cause specific dampening of cellular responses to stimuli such as hormones, neurotransmitters, or sensory signals. Arrestin beta 1 is a cytosolic protein and acts as a cofactor in the beta-adrenergic receptor kinase (BARK) mediated desensitization of beta-adrenergic receptors. Besides the central nervous system, it is expressed at high levels in peripheral blood leukocytes, and thus the BARK/beta-arrestin system is believed to play a major role in regulating receptor-mediated immune functions. Alternatively spliced transcripts encoding different isoforms of arrestin beta 1 have been described. [provided by RefSeq, Jan 2011],
      • Function:
      • function:Regulates beta-adrenergic receptor function. Beta-arrestins seem to bind phosphorylated beta-adrenergic receptors, thereby causing a significant impairment of their capacity to activate G(S) proteins.,online information:Arrestin entry,similarity:Belongs to the arrestin family.,
      • Subcellular Location:
      • Cytoplasm. Nucleus. Cell membrane. Membrane, clathrin-coated pit . Cell projection, pseudopodium . Cytoplasmic vesicle. Translocates to the plasma membrane and colocalizes with antagonist-stimulated GPCRs. The monomeric form is predominantly located in the nucleus. The oligomeric form is located in the cytoplasm. Translocates to the nucleus upon stimulation of OPRD1 (By similarity). .
      • Expression:
      • Brain,Peripheral blood,Uterus,
      • Products Images
      • Western blot analysis of lysates from Jurkat and HUVEC cells, using ARRB1 Antibody. The lane on the right is blocked with the synthesized peptide.
      • Immunohistochemical analysis of paraffin-embedded human brain tumor. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).