ARF4 Polyclonal Antibody

    • Catalog No.:YT0308
    • Applications:WB,IHC-p,IF(paraffin section),ELISA
    • Reactivity:Human,Rat,Mouse,
      • Gene Name:
      • ARF4
      • Protein Name:
      • ADP-ribosylation factor 4
      • Human Gene Id:
      • 378
      • Human Swiss Prot No:
      • P18085
      • Mouse Swiss Prot No:
      • P61750
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human ARF4. AA range:71-120
      • Specificity:
      • ARF4 Polyclonal Antibody detects endogenous levels of ARF4 protein.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Polyclonal Rabbit
      • Dilution:
      • Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -20°C/1 year
      • Other Name:
      • ARF4; ARF2; ADP-ribosylation factor 4
      • MolecularWeight(Da):
      • 20511
      • Observed Band(KD):
      • 25
      • Background:
      • ADP ribosylation factor 4(ARF4) Homo sapiens This gene is a member of the human ARF gene family whose members encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The gene products include 5 ARF proteins and 11 ARF-like proteins and constitute one family of the RAS superfamily. The ARF proteins are categorized as class I, class II and class III; this gene is a class II member. The members of each class share a common gene organization. The ARF4 gene spans approximately 12kb and contains six exons and five introns. This gene is the most divergent member of the human ARFs. Conflicting map positions at 3p14 or 3p21 have been reported for this gene. [provided by RefSeq, Jul 2008],
      • Function:
      • caution:Was originally thought to be ARF2.,function:GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus.,similarity:Belongs to the small GTPase superfamily. Arf family.,
      • Subcellular Location:
      • intracellular,Golgi apparatus,cytosol,membrane,integral component of membrane,extracellular matrix,ruffle membrane,dendritic spine,extracellular exosome,
      • Expression:
      • Bone marrow,Brain,Cerebellum,Placenta,Skin,Urinary bladder,
      • Products Images
      • Immunohistochemical analysis of paraffin-embedded Human lung cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
      • Immunohistochemical analysis of paraffin-embedded Human lung cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
      • Western blot analysis of lysates from LOVO, K562, and HT-29 cells, using ARF4 Antibody. The lane on the right is blocked with the synthesized peptide.