A-Raf Polyclonal Antibody

    • Catalog No.:YT0303
    • Applications:WB;IHC;IF;ELISA
    • Reactivity:Human;Mouse;Rat
      • Target:
      • A-Raf
      • Fields:
      • >>EGFR tyrosine kinase inhibitor resistance;>>Endocrine resistance;>>MAPK signaling pathway;>>ErbB signaling pathway;>>FoxO signaling pathway;>>Vascular smooth muscle contraction;>>Natural killer cell mediated cytotoxicity;>>Long-term potentiation;>>Serotonergic synapse;>>Long-term depression;>>Regulation of actin cytoskeleton;>>Insulin signaling pathway;>>Progesterone-mediated oocyte maturation;>>Parathyroid hormone synthesis, secretion and action;>>Alzheimer disease;>>Pathways of neurodegeneration - multiple diseases;>>Alcoholism;>>Hepatitis C;>>Hepatitis B;>>Pathways in cancer;>>Proteoglycans in cancer;>>Chemical carcinogenesis - reactive oxygen species;>>Colorectal cancer;>>Renal cell carcinoma;>>Pancreatic cancer;>>Endometrial cancer;>>Glioma;>>Prostate cancer;>>Melanoma;>>Bladder cancer;>>Chronic myeloid leukemia;>>Acute myeloid leukemia;>>Non-small cell lung cancer;>>Breast cancer;>>Hepatocellular carcinoma;>>Gastric cancer
      • Gene Name:
      • ARAF
      • Protein Name:
      • Serine/threonine-protein kinase A-Raf
      • Human Gene Id:
      • 369
      • Human Swiss Prot No:
      • P10398
      • Mouse Swiss Prot No:
      • P04627
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human A-RAF. AA range:276-325
      • Specificity:
      • A-Raf Polyclonal Antibody detects endogenous levels of A-Raf protein.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Polyclonal, Rabbit,IgG
      • Dilution:
      • WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Other Name:
      • ARAF;ARAF1;PKS;PKS2;Serine/threonine-protein kinase A-Raf;Proto-oncogene A-Raf;Proto-oncogene A-Raf-1;Proto-oncogene Pks
      • Observed Band(KD):
      • 68kD
      • Background:
      • This proto-oncogene belongs to the RAF subfamily of the Ser/Thr protein kinase family, and maybe involved in cell growth and development. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jan 2012],
      • Function:
      • catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Binds 2 zinc ions per subunit.,function:Involved in the transduction of mitogenic signals from the cell membrane to the nucleus.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.,similarity:Contains 1 phorbol-ester/DAG-type zinc finger.,similarity:Contains 1 protein kinase domain.,similarity:Contains 1 RBD (Ras-binding) domain.,subunit:Interacts with TH1L/NELFD.,tissue specificity:Predominantly in urogenital tissues.,
      • Subcellular Location:
      • intracellular,mitochondrion,cytosol,
      • Expression:
      • Predominantly in urogenital tissues.
      • Products Images
      • Western blot analysis in MCF-7 cells transfected with siA-Raf. Cancer Letters 355 (2014) 288–296
      • Western Blot analysis of various cells using A-Raf Polyclonal Antibody
      • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using A-RAF Antibody. The picture on the right is blocked with the synthesized peptide.