Annexin I Polyclonal Antibody

    • Catalog No.:YT0234
    • Applications:WB,IHC-p,IF(paraffin section),ELISA
    • Reactivity:Human,Mouse,Rat
      • Gene Name:
      • ANXA1
      • Protein Name:
      • Annexin A1
      • Human Gene Id:
      • 301
      • Human Swiss Prot No:
      • P04083
      • Mouse Swiss Prot No:
      • P10107
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human Annexin I. AA range:97-146
      • Specificity:
      • Annexin I Polyclonal Antibody detects endogenous levels of Annexin I protein.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Rabbit
      • Dilution:
      • Western Blot: 1/500 - 1/2000.IHC-p:1:50-300 ELISA: 1/20000. Not yet tested in other applications.
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -20°C/1 year
      • Other Name:
      • ANXA1; ANX1; LPC1; Annexin A1; Annexin I; Annexin-1; Calpactin II; Calpactin-2; Chromobindin-9; Lipocortin I; Phospholipase A2 inhibitory protein; p35
      • MolecularWeight(Da):
      • 38714
      • Observed Band(KD):
      • 38
      • Background:
      • annexin A1(ANXA1) Homo sapiens This gene encodes a membrane-localized protein that binds phospholipids. This protein inhibits phospholipase A2 and has anti-inflammatory activity. Loss of function or expression of this gene has been detected in multiple tumors. [provided by RefSeq, Dec 2014],
      • Function:
      • domain:A pair of annexin repeats may form one binding site for calcium and phospholipid.,function:Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis. This protein regulates phospholipase A2 activity. It seems to bind from two to four calcium ions with high affinity.,PTM:Phosphorylated by protein kinase C, epidermal growth factor receptor/kinase and TRPM7. Phosphorylation results in loss of the inhibitory activity.,similarity:Belongs to the annexin family.,similarity:Contains 1 annexin repeat.,similarity:Contains 2 annexin repeats.,similarity:Contains 4 annexin repeats.,subcellular location:Found in the cilium, nucleus and basolateral cell membrane of ciliated cells in the tracheal endothelium (By similarity). Found in the cytoplasm of type II pneumocytes and alveolar macrophages.,subunit:Homodimer in placenta (20%); linked by transglutamylat
      • Subcellular Location:
      • cornified envelope,phagocytic cup,extracellular region,extracellular space,nucleus,nucleoplasm,cytoplasm,endosome,plasma membrane,cell-cell adherens junction,focal adhesion,cell surface,basolateral plasma membrane,
      • Expression:
      • Cervix,Epithelium,Lung,Placenta,
      • Products Images
      • Western Blot analysis of various cells using primary antibody diluted at 1:1000(4°C overnight). Secondary antibody:Goat Anti-rabbit IgG IRDye 800( diluted at 1:5000, 25°C, 1 hour). Cell lysate was extracted by Minute™ Plasma Membrane Protein Isolation and Cell Fractionation Kit(SM-005, Inventbiotech,MN,USA).
      • Western Blot analysis of various cells using Annexin I Polyclonal Antibody diluted at 1:2000
      • Western Blot analysis of HeLa cells using Annexin I Polyclonal Antibody diluted at 1:2000
      • Immunohistochemistry analysis of Annexin I antibody in paraffin-embedded human brain tissue.
      • Western blot analysis of lysate from HeLa cells, using Annexin I antibody.