AMPKγ2 Polyclonal Antibody

    • Catalog No.:YT0222
    • Applications:WB;IHC;IF;ELISA
    • Reactivity:Human;Mouse
      • Target:
      • AMPKγ2
      • Fields:
      • >>FoxO signaling pathway;>>AMPK signaling pathway;>>Longevity regulating pathway;>>Longevity regulating pathway - multiple species;>>Apelin signaling pathway;>>Tight junction;>>Circadian rhythm;>>Thermogenesis;>>Insulin signaling pathway;>>Adipocytokine signaling pathway;>>Oxytocin signaling pathway;>>Glucagon signaling pathway;>>Insulin resistance;>>Non-alcoholic fatty liver disease;>>Alcoholic liver disease;>>Hypertrophic cardiomyopathy
      • Gene Name:
      • PRKAG2
      • Protein Name:
      • 5'-AMP-activated protein kinase subunit gamma-2
      • Human Swiss Prot No:
      • Q9UGJ0
      • Mouse Swiss Prot No:
      • Q91WG5
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human PRKAG2. AA range:1-50
      • Specificity:
      • AMPKγ2 Polyclonal Antibody detects endogenous levels of AMPKγ2 protein.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Polyclonal, Rabbit,IgG
      • Dilution:
      • WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Other Name:
      • PRKAG2;5'-AMP-activated protein kinase subunit gamma-2;AMPK gamma2;AMPK subunit gamma-2;H91620p
      • Observed Band(KD):
      • 65kD
      • Background:
      • AMP-activated protein kinase (AMPK) is a heterotrimeric protein composed of a catalytic alpha subunit, a noncatalytic beta subunit, and a noncatalytic regulatory gamma subunit. Various forms of each of these subunits exist, encoded by different genes. AMPK is an important energy-sensing enzyme that monitors cellular energy status and functions by inactivating key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This gene is a member of the AMPK gamma subunit family. Mutations in this gene have been associated with Wolff-Parkinson-White syndrome, familial hypertrophic cardiomyopathy, and glycogen storage disease of the heart. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jan 2015],
      • Function:
      • disease:Defects in PRKAG2 are a cause of cardiomyopathy familial hypertrophic with Wolff-Parkinson-White syndrome (CHMWPWS) [MIM:600858]. HCM due to PRKAG2 mutations is probably due to polysaccharide storage in the heart. Defects in PRKAG2 may not be a frequent cause of HCM where no features of pre-excitation are found in affected individuals.,disease:Defects in PRKAG2 are a cause of glycogen storage disease of heart lethal congenital (GSDH) [MIM:261740]; also known as phosphorylase kinase deficiency of heart or congenital nonlysosomal cardiac glycogenosis. GSDH is a rare disease which leads to death within a few weeks to a few months after birth, through heart failure and respiratory compromise.,disease:Defects in PRKAG2 are the cause of Wolff-Parkinson-White syndrome (WPWS) [MIM:194200]; also known as preexcitation syndrome. It is the second most common cause of paroxysmal supraventric
      • Subcellular Location:
      • extracellular space,nucleoplasm,cytosol,nucleotide-activated protein kinase complex,
      • Expression:
      • Isoform B is ubiquitously expressed except in liver and thymus. The highest level is detected in heart with abundant expression in placenta and testis.
      • Products Images
      • Western Blot analysis of K562 using Antibody diluted at 1:1000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
      • Immunofluorescence analysis of A549 cells, using PRKAG2 Antibody. The picture on the right is blocked with the synthesized peptide.
      • Immunohistochemistry analysis of paraffin-embedded human heart tissue, using PRKAG2 Antibody. The picture on the right is blocked with the synthesized peptide.