Actinin-α3 Polyclonal Antibody

    • Catalog No.:YT0103
    • Applications:WB,IF/ICC,ELISA
    • Reactivity:Human,Mouse,Rat
      • Gene Name:
      • ACTN3
      • Protein Name:
      • Alpha-actinin-3
      • Human Gene Id:
      • 89
      • Human Swiss Prot No:
      • Q08043
      • Mouse Swiss Prot No:
      • O88990
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human ACTN3. AA range:1-50
      • Specificity:
      • Actinin-α3 Polyclonal Antibody detects endogenous levels of Actinin-α3 protein.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Rabbit
      • Dilution:
      • Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -20°C/1 year
      • Other Name:
      • ACTN3; Alpha-actinin-3; Alpha-actinin skeletal muscle isoform 3; F-actin cross-linking protein
      • MolecularWeight(Da):
      • 103294
      • Observed Band(KD):
      • 103
      • Background:
      • actinin alpha 3 (gene/pseudogene)(ACTN3) Homo sapiens This gene encodes a member of the alpha-actin binding protein gene family. The encoded protein is primarily expressed in skeletal muscle and functions as a structural component of sarcomeric Z line. This protein is involved in crosslinking actin containing thin filaments. An allelic polymorphism in this gene results in both coding and non-coding variants; the reference genome represents the coding allele. The non-functional allele of this gene is associated with elite athlete status. [provided by RefSeq, Feb 2014],
      • Function:
      • function:F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.,polymorphism:About 18% of the world population lack a functional ACTN3 due to a stop codon polymorphism at position 577. The absence of a functional ACTN3 expression is not correlated with a disease state.,sequence caution:According to the human genome assembly there is a stop codon in position 577 which is only found in 18% of the world population.,similarity:Belongs to the alpha-actinin family.,similarity:Contains 1 actin-binding domain.,similarity:Contains 2 CH (calponin-homology) domains.,similarity:Contains 2 EF-hand domains.,similarity:Contains 4 spectrin repeats.,subunit:Homodimer; antiparallel. Also forms heterodimers with ACTN2. Interacts with MYOZ1.,tissue specificity:Expressed only in a subset of type 2 skeletal muscle fibers.,
      • Subcellular Location:
      • intracellular,cytosol,actin filament,brush border,focal adhesion,Z disc,pseudopodium,extracellular exosome,
      • Expression:
      • PCR rescued clones,Skeletal muscle,
      • Products Images
      • Western Blot analysis of various cells using Actinin-α3 Polyclonal Antibody
      • Western Blot analysis of HT29 cells using Actinin-α3 Polyclonal Antibody
      • Western blot analysis of lysates from HT-29 cells, using ACTN3 Antibody. The lane on the right is blocked with the synthesized peptide.