14-3-3 η Polyclonal Antibody

    • Catalog No.:YT0009
    • Applications:WB,IHC-p,IF,ELISA
    • Reactivity:Human,Mouse,Rat
      • Gene Name:
      • YWHAH
      • Protein Name:
      • 14-3-3 protein eta
      • Human Gene Id:
      • 7533
      • Human Swiss Prot No:
      • Q04917
      • Mouse Swiss Prot No:
      • P68510
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human 14-3-3 eta. AA range:51-100
      • Specificity:
      • 14-3-3 η Polyclonal Antibody detects endogenous levels of 14-3-3 η protein.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Rabbit
      • Dilution:
      • Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -20°C/1 year
      • Other Name:
      • YWHAH; YWHA1; 14-3-3 protein eta; Protein AS1
      • MolecularWeight(Da):
      • 28082
      • Observed Band(KD):
      • 42
      • Background:
      • tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein eta(YWHAH) Homo sapiens This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and bovine orthologs. This gene contains a 7 bp repeat sequence in its 5' UTR, and changes in the number of this repeat have been associated with early-onset schizophrenia and psychotic bipolar disorder. [provided by RefSeq, Jun 2009],
      • Function:
      • function:Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathway. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.,similarity:Belongs to the 14-3-3 family.,subunit:Homodimer (By similarity). Interacts with many nuclear hormone receptors and cofactors including AR, ESR1, ESR2, MC2R, NR3C1, NRIP1, PPARBP and THRA. Interacts with ABL1 (phosphorylated form); the interaction retains it in the cytoplasm. Interacts with RGNEF and PCTK1 (By similarity). Weakly interacts with CDKN1B.,tissue specificity:Expressed mainly in the brain and present in other tissues albeit at lower levels.,
      • Subcellular Location:
      • cytoplasm,mitochondrion,cytosol,plasma membrane,intercalated disc,cytoplasmic vesicle membrane,extracellular exosome,
      • Expression:
      • Brain,Keratinocyte,Lymph,Pl
      • Products Images
      • Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4°,overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
      • Immunofluorescence analysis of HeLa cells, using 14-3-3 eta Antibody. The picture on the right is blocked with the synthesized peptide.
      • Western blot analysis of lysates from Jurkat cells, using 14-3-3 eta Antibody. The lane on the right is blocked with the synthesized peptide.