• Target：
• MLK3

• Fields：
• >>MAPK signaling pathway;>>Non-alcoholic fatty liver disease

• Gene Name：
• MAP3K11 MLK3 PTK1 SPRK

• Protein Name：
• MLK3 (Phospho Thr277+Ser281)

• Human Gene Id：
• 4296

• Human Swiss Prot No：
• Q16584

• Mouse Gene Id：
• 26403

• Mouse Swiss Prot No：
• Q80XI6

• Rat Gene Id：
• 309168

• Rat Swiss Prot No：
• Q66HA1

• Immunogen：
• Synthesized peptide derived from human MLK3 (Phospho Thr277+Ser281)

• Specificity：
• This antibody detects endogenous levels of Human,Mouse,Rat MLK3 (Phospho Thr277+Ser281)

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500-2000;IHC 1:50-300; ELISA 2000-20000

• Purification：
• The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• Mitogen-activated protein kinase kinase kinase 11 (EC 2.7.11.25;Mixed lineage kinase 3;Src-homology 3 domain-containing proline-rich kinase)

• Molecular Weight(Da)：
• 93kD

• Background：
• The protein encoded by this gene is a member of the serine/threonine kinase family. This kinase contains a SH3 domain and a leucine zipper-basic motif. This kinase preferentially activates MAPK8/JNK kinase, and functions as a positive regulator of JNK signaling pathway. This kinase can directly phosphorylate, and activates IkappaB kinase alpha and beta, and is found to be involved in the transcription activity of NF-kappaB mediated by Rho family GTPases and CDC42. [provided by RefSeq, Jul 2008],

• Function：
• catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,enzyme regulation:Homodimerization via the leucine zipper domains is required for autophosphorylation and subsequent activation.,function:Activates the JUN N-terminal pathway. Required for serum-stimulated cell proliferation and for mitogen and cytokine activation of MAPK14 (p38), MAPK3 (ERK) and MAPK8 (JNK1). Plays a role in mitogen-stimulated phosphorylation and activation of BRAF, but does not phosphorylate BRAF directly. Influences microtubule organization during the cell cycle.,PTM:Autophosphorylation on serine and threonine residues within the activation loop plays a role in enzyme activation. Thr-277 is likely to be the main autophosphorylation site. Phosphorylation of Ser-555 and Ser-556 is induced by CDC42.,similarity:Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP

• Subcellular Location：
• Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Location is cell cycle dependent.

• Expression：
• Expressed in a wide variety of normal and neoplastic tissues including fetal lung, liver, heart and kidney, and adult lung, liver, heart, kidney, placenta, skeletal muscle, pancreas and brain.

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