• Target：
• RIP

• Fields：
• >>NF-kappa B signaling pathway;>>Apoptosis;>>Necroptosis;>>Toll-like receptor signaling pathway;>>NOD-like receptor signaling pathway;>>RIG-I-like receptor signaling pathway;>>Cytosolic DNA-sensing pathway;>>TNF signaling pathway;>>Alcoholic liver disease;>>Pathogenic Escherichia coli infection;>>Shigellosis;>>Salmonella infection;>>Hepatitis C;>>Human cytomegalovirus infection;>>Epstein-Barr virus infection;>>Human immunodeficiency virus 1 infection

• Gene Name：
• RIPK1 RIP RIP1

• Protein Name：
• RIP (Ser166)

• Human Gene Id：
• 8737

• Human Swiss Prot No：
• Q13546

• Mouse Gene Id：
• 19766

• Mouse Swiss Prot No：
• Q60855

• Immunogen：
• Synthesized phosho peptide around human RIP (Ser166)

• Specificity：
• This antibody detects endogenous levels of Human RIP (phospho-Ser166)

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500-2000;IHC 1:50-300

• Purification：
• The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• Receptor-interacting serine/threonine-protein kinase 1 (EC 2.7.11.1) (Cell death protein RIP) (Receptor-interacting protein 1) (RIP-1) (Serine/threonine-protein kinase RIP)

• Observed Band(KD)：
• 73kD

• Function：
• catalytic activity:ATP + a protein = ADP + a phosphoprotein.,function:Promotes apoptosis and activation of NF-kappa-B. Required for TNFRSF1A mediated activation of NF-kappa-B.,PTM:Autophosphorylated on serine and threonine residues.,PTM:Proteolytically cleaved by caspase-8 during TNF-induced apoptosis. Cleavage abolishes NF-kappa-B activation and enhances pro-apototic signaling through the TRADD-FADD interaction.,similarity:Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.,similarity:Contains 1 death domain.,similarity:Contains 1 protein kinase domain.,subunit:Binds to the death domain of TNFRSF6 and TRADD. Is recruited by TRADD to TNFRSF1A in a TNF-dependent process. Binds RIPK3, UBCE7IP1 isoform 3 (ZIN), EGFR, IKBKG, TRAF1, TRAF2 and TRAF3. Interacts with BNLF1. Interacts with SQSTM1 upon TNF-alpha stimulation. May interacts with MAVS/IPS1.,

• Subcellular Location：
• Cytoplasm . Cell membrane .

• Expression：
• Leukemic T-cell,T-cell,Umbilical vein endothelial cell,

Salmonella pSLT-encoded effector SpvB promotes RIPK3-dependent necroptosis in intestinal epithelial cells Cell Death Discov. 2022 Feb;8(1):1-11. WB Human

TNF-α preconditioned UCMSCs-derived EVs enhance the inhibition of necroptosis of acinar cells in SAP. Tissue Engineering Part A Prof. Zhenshun Song WB Rat 1:1000 AR42J cell

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