• Target：
• NIFK

• Gene Name：
• MKI67IP

• Protein Name：
• MKI67 FHA domain-interacting nucleolar phosphoprotein

• Human Gene Id：
• 84365

• Human Swiss Prot No：
• Q9BYG3

• Mouse Gene Id：
• 67949

• Mouse Swiss Prot No：
• Q91VE6

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human NIFK around the phosphorylation site of Thr234. AA range:200-249

• Specificity：
• Phospho-NIFK (T234) Polyclonal Antibody detects endogenous levels of NIFK protein only when phosphorylated at T234.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:40000. Not yet tested in other applications.

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• MKI67IP;NIFK;NOPP34;MKI67 FHA domain-interacting nucleolar phosphoprotein;Nucleolar phosphoprotein Nopp34;Nucleolar protein interacting with the FHA domain of pKI-67;hNIFK

• Molecular Weight(Da)：
• 34kD

• Background：
• This gene encodes a protein that interacts with the forkhead-associated domain of the Ki-67 antigen. The encoded protein may bind RNA and may play a role in mitosis and cell cycle progression. Multiple pseudogenes exist on chromosomes 5, 10, 12, 15, and 19.[provided by RefSeq, Jan 2009],

• Function：
• PTM:Sequentially phosphorylated on Thr-238, Thr-234 and Ser-230. Thr-234 is phosphorylated only when Thr-238 is phosphorylated. Likewise, phosphorylation at Ser-230 requires that Thr-234 and Thr-238 are phosphorylated. Phosphorylation enhances MKI67 binding.,similarity:Contains 1 RRM (RNA recognition motif) domain.,subcellular location:Localizes to mitotic chromosomes in conjunction with MKI67.,subunit:Binds to the FHA domain of MKI67; this interaction is enhanced in mitosis.,

• Subcellular Location：
• Nucleus, nucleolus. Chromosome. Localizes to mitotic chromosomes in conjunction with MKI67.

• Expression：
• Brain,Cervix carcinoma,Epithelium,Lung,Plac

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