HSF1 (phospho Thr142) Polyclonal Antibody

    • Catalog No.:YP1026
    • Applications:IHC;IF;ELISA
    • Reactivity:Human;Mouse
      • Target:
      • HSF1
      • Fields:
      • >>Legionellosis
      • Gene Name:
      • HSF1
      • Protein Name:
      • Heat shock factor protein 1
      • Human Gene Id:
      • 3297
      • Human Swiss Prot No:
      • Q00613
      • Mouse Swiss Prot No:
      • P38532
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human HSF1 around the phosphorylation site of Thr142. AA range:108-157
      • Specificity:
      • Phospho-HSF1 (T142) Polyclonal Antibody detects endogenous levels of HSF1 protein only when phosphorylated at T142.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Polyclonal, Rabbit,IgG
      • Dilution:
      • IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Other Name:
      • HSF1;HSTF1;Heat shock factor protein 1;HSF 1;Heat shock transcription factor 1;HSTF 1
      • Molecular Weight(Da):
      • 31kD
      • Background:
      • heat shock transcription factor 1(HSF1) Homo sapiens The product of this gene is a transcription factor that is rapidly induced after temperature stress and binds heat shock promoter elements (HSE). This protein plays a role in the regulation of lifespan. Expression of this gene is repressed by phsphorylation, which promotes binding by heat shock protein 90. [provided by RefSeq, Aug 2016],
      • Function:
      • function:DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.,PTM:Phosphorylated on multiple serine residues, a subset of which are involved in stress-related regulation of transcription activation. Constitutive phosphorylation represses transcriptional activity at normal temperatures. Levels increase on specific residues heat-shock and enhance HSF1 transactivation activity. Phosphorylation on Ser-307 derepresses activation on heat-stress and in combination with Ser-303 phosphorylation appears to be involved in recovery after heat-stress. Phosphorylated on Ser-230 by CAMK2, in vitro. Cadmium also enhances phosphorylation at this site. Phosphorylation on Ser-303 is a prerequisite for HSF1 sumoylation. Phosphorylation on Ser-121 inhibits transacti
      • Subcellular Location:
      • Nucleus . Cytoplasm . Nucleus, nucleoplasm . Cytoplasm, perinuclear region . Cytoplasm, cytoskeleton, spindle pole . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Chromosome, centromere, kinetochore . The monomeric form is cytoplasmic in unstressed cells (PubMed:8455624, PubMed:26159920). Predominantly nuclear protein in both unstressed and heat shocked cells (PubMed:10413683, PubMed:10359787). Translocates in the nucleus upon heat shock (PubMed:8455624). Nucleocytoplasmic shuttling protein (PubMed:26159920). Colocalizes with IER5 in the nucleus (PubMed:27354066). Colocalizes with BAG3 to the nucleus upon heat stress (PubMed:8455624, PubMed:26159920). Localizes in subnuclear granules called nuclear stress bodies (nSBs) upon heat shock (PubMed:11447121, PubMed:1151455
      • Expression:
      • Adipose tissue,Brain,Epithelium,Muscle,
      • Products Images
      • Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using HSF1 (Phospho-Thr142) Antibody
      • Immunohistochemistry analysis of paraffin-embedded human brain, using HSF1 (Phospho-Thr142) Antibody. The picture on the right is blocked with the phospho peptide.