Bcr (phospho Tyr360) Polyclonal Antibody

    • Catalog No.:YP0905
    • Applications:WB;IHC;IF;ELISA
    • Reactivity:Human;Mouse;Monkey
      • Target:
      • Bcr
      • Fields:
      • >>Pathways in cancer;>>Chronic myeloid leukemia
      • Gene Name:
      • BCR
      • Protein Name:
      • Breakpoint cluster region protein
      • Human Gene Id:
      • 613
      • Human Swiss Prot No:
      • P11274
      • Mouse Swiss Prot No:
      • Q6PAJ1
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human Bcr around the phosphorylation site of Tyr360. AA range:331-380
      • Specificity:
      • Phospho-Bcr (Y360) Polyclonal Antibody detects endogenous levels of Bcr protein only when phosphorylated at Y360.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Polyclonal, Rabbit,IgG
      • Dilution:
      • WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:40000. Not yet tested in other applications.
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Other Name:
      • BCR;BCR1;D22S11;Breakpoint cluster region protein;Renal carcinoma antigen NY-REN-26
      • Observed Band(KD):
      • 142kD
      • Background:
      • A reciprocal translocation between chromosomes 22 and 9 produces the Philadelphia chromosome, which is often found in patients with chronic myelogenous leukemia. The chromosome 22 breakpoint for this translocation is located within the BCR gene. The translocation produces a fusion protein which is encoded by sequence from both BCR and ABL, the gene at the chromosome 9 breakpoint. Although the BCR-ABL fusion protein has been extensively studied, the function of the normal BCR gene product is not clear. The protein has serine/threonine kinase activity and is a GTPase-activating protein for p21rac. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008],
      • Function:
      • catalytic activity:ATP + a protein = ADP + a phosphoprotein.,disease:A chromosomal aberration involving BCR is a cause of chronic myeloid leukemia (CML) [MIM:608232]. Translocation t(9;22)(q34;q11) with ABL1. The translocation produces a BCR-ABL found also in acute myeloid leukemia (AML) and acute lymphoblastic leukemia (ALL).,domain:The DH domain is involved in interaction with CCPG1.,domain:The region involved in binding to ABL1 SH2-domain is rich in serine residues and needs to be Ser/Thr phosphorylated prior to SH2 binding. This region is essential for the activation of the ABL1 tyrosine kinase and transforming potential of the chimeric BCR-ABL oncogene.,function:GTPase-activating protein for RAC1 and CDC42. Promotes the exchange of RAC or CDC42-bound GDP by GTP, thereby activating them. Displays serine/threonine kinase activity.,PTM:Autophosphorylated.,similarity:Contains 1 C2 domai
      • Subcellular Location:
      • Cell junction, synapse, postsynaptic density . Cell projection, dendritic spine . Cell projection, axon . Cell junction, synapse .
      • Expression:
      • Brain,Epithelium,Platelet,Renal cell carcinoma,T-cell,
      • Products Images
      • Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Bcr (Phospho-Tyr360) Antibody
      • Immunofluorescence analysis of NIH/3T3 cells, using Bcr (Phospho-Tyr360) Antibody. The picture on the right is blocked with the phospho peptide.
      • Immunohistochemistry analysis of paraffin-embedded human brain, using Bcr (Phospho-Tyr360) Antibody. The picture on the right is blocked with the phospho peptide.
      • Western blot analysis of lysates from COS7 cells, using Bcr (Phospho-Tyr360) Antibody. The lane on the right is blocked with the phospho peptide.