CtBP1 (phospho Ser422) Polyclonal Antibody

    • Catalog No.:YP0815
    • Applications:WB;IHC;IF;ELISA
    • Reactivity:Human;Mouse;Rat
      • Target:
      • CtBP1
      • Fields:
      • >>Wnt signaling pathway;>>Notch signaling pathway;>>Pathways in cancer;>>Chronic myeloid leukemia
      • Gene Name:
      • CTBP1
      • Protein Name:
      • C-terminal-binding protein 1
      • Human Gene Id:
      • 1487
      • Human Swiss Prot No:
      • Q13363
      • Mouse Swiss Prot No:
      • O88712
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human CtBP1 around the phosphorylation site of Ser422. AA range:388-437
      • Specificity:
      • Phospho-CtBP1 (S422) Polyclonal Antibody detects endogenous levels of CtBP1 protein only when phosphorylated at S422.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Polyclonal, Rabbit,IgG
      • Dilution:
      • WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Other Name:
      • CTBP1;CTBP;C-terminal-binding protein 1;CtBP1
      • Observed Band(KD):
      • 48kD
      • Background:
      • This gene encodes a protein that binds to the C-terminus of adenovirus E1A proteins. This phosphoprotein is a transcriptional repressor and may play a role during cellular proliferation. This protein and the product of a second closely related gene, CTBP2, can dimerize. Both proteins can also interact with a polycomb group protein complex which participates in regulation of gene expression during development. Alternative splicing of transcripts from this gene results in multiple transcript variants. [provided by RefSeq, Jul 2008],
      • Function:
      • cofactor:NAD. Required for efficient interaction with E1A. Cofactor binding induces a conformation change.,function:Involved in controlling the equilibrium between tubular and stacked structures in the Golgi complex (By similarity). Co-repressor targeting diverse transcription regulators such as GLIS2. Has dehydrogenase activity.,PTM:ADP-ribosylated; when cells are exposed to brefeldin-A (BFA).,PTM:Sumoylation on Lys-428 is promoted by the E3 SUMO-protein ligase CBX4.,PTM:The level of phosphorylation appears to be regulated during the cell cycle. Phosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylation by HIPK2 on Ser-422 induces proteasomal degradation.,similarity:Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.,subunit:Interacts with the C-terminus of adenovirus E1A protein, ELK3 and CTIP via their consensus motif P-X-[DNS]-L-[STVA]. Can form homodime
      • Subcellular Location:
      • Cytoplasm . Nucleus .
      • Expression:
      • Expressed in germinal center B-cells.
      • Products Images
      • Western Blot analysis of JK cells using Phospho-CtBP1 (S422) Polyclonal Antibody
      • Immunohistochemistry analysis of paraffin-embedded human brain, using CtBP1 (Phospho-Ser422) Antibody. The picture on the right is blocked with the phospho peptide.
      • Western blot analysis of lysates from Jurkat cells treated with TNF 20ng/ml 30' and Jurkat cells treated with UV 15', using CtBP1 (Phospho-Ser422) Antibody. The lane on the right is blocked with the phospho peptide.