p130 Cas (phospho Tyr249) Polyclonal Antibody

    • Catalog No.:YP0698
    • Applications:WB,IHC-p,IF(paraffin section),ELISA
    • Reactivity:Human,Mouse,Rat
      • Gene Name:
      • BCAR1
      • Protein Name:
      • Breast cancer anti-estrogen resistance protein 1
      • Human Gene Id:
      • 9564
      • Human Swiss Prot No:
      • P56945
      • Mouse Swiss Prot No:
      • Q61140
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human p130 Cas around the phosphorylation site of Tyr249. AA range:215-264
      • Specificity:
      • Phospho-p130 Cas (Y249) Polyclonal Antibody detects endogenous levels of p130 Cas protein only when phosphorylated at Y249.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Rabbit
      • Dilution:
      • IF(paraffin section) 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -20°C/1 year
      • Other Name:
      • BCAR1; CAS; CASS1; CRKAS; Breast cancer anti-estrogen resistance protein 1; CRK-associated substrate; Cas scaffolding protein family member 1; p130cas
      • MolecularWeight(Da):
      • 93362
      • Observed Band(KD):
      • 130
      • Background:
      • BCAR1, Cas family scaffolding protein(BCAR1) Homo sapiens BCAR1, or CAS, is an Src (MIM 190090) family kinase substrate involved in various cellular events, including migration, survival, transformation, and invasion (Sawada et al., 2006 [PubMed 17129785]).[supplied by OMIM, May 2009],
      • Function:
      • domain:A serine-rich region promotes activation of the serum response element (SRE).,domain:Contains a central domain (substrate domain) containing multiple potential SH2-binding sites and a C-terminal domain containing a divergent helix-loop-helix (HLH) motif. The SH2-binding sites putatively bind CRK, NCK and ABL SH2 domains. The HLH motif is absolutely required for the induction of pseudohyphal growth in yeast and mediates heterodimerization with CASL.,domain:The SH3 domain is necessary for the localization of the protein to focal adhesions and interacts with one proline-rich region of focal adhesion kinase 1.,function:Docking protein which plays a central coordinating role for tyrosine-kinase-based signaling related to cell adhesion. Implicated in induction of cell migration. Overexpression confers antiestrogen resistance on breast cancer cells.,PTM:Focal adhesion kinase 1 phosphoryl
      • Subcellular Location:
      • ruffle,nucleolus,cytoplasm,cytosol,plasma membrane,focal adhesion,actin cytoskeleton,lamellipodium,
      • Expression:
      • Caudate nucleus,Cerebellum,Cornea,Epithelium,Kidney,Lung,Mammary ca
      • Products Images
      • Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using p130 Cas (Phospho-Tyr249) Antibody
      • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using p130 Cas (Phospho-Tyr249) Antibody. The picture on the right is blocked with the phospho peptide.
      • Western blot analysis of lysates from HepG2 cells treated with EGF 200ng/ml 30' and A549 cells treated with PMA 125ng/ml 30', using p130 Cas (Phospho-Tyr249) Antibody. The lane on the right is blocked with the phospho peptide.