GR (phospho Ser226) Polyclonal Antibody

    • Catalog No.:YP0634
    • Applications:WB,IHC-p,IF(paraffin section),ELISA
    • Reactivity:Human,Mouse,Rat
      • Gene Name:
      • NR3C1
      • Protein Name:
      • Glucocorticoid receptor
      • Human Gene Id:
      • 2908
      • Human Swiss Prot No:
      • P04150
      • Mouse Swiss Prot No:
      • P06537
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human GR around the phosphorylation site of Ser226. AA range:201-250
      • Specificity:
      • Phospho-GR (S226) Polyclonal Antibody detects endogenous levels of GR protein only when phosphorylated at S226.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Rabbit
      • Dilution:
      • IF(paraffin section) 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -20°C/1 year
      • Other Name:
      • NR3C1; GRL; Glucocorticoid receptor; GR; Nuclear receptor subfamily 3 group C member 1
      • MolecularWeight(Da):
      • 85659
      • Observed Band(KD):
      • 86
      • Background:
      • nuclear receptor subfamily 3 group C member 1(NR3C1) Homo sapiens This gene encodes glucocorticoid receptor, which can function both as a transcription factor that binds to glucocorticoid response elements in the promoters of glucocorticoid responsive genes to activate their transcription, and as a regulator of other transcription factors. This receptor is typically found in the cytoplasm, but upon ligand binding, is transported into the nucleus. It is involved in inflammatory responses, cellular proliferation, and differentiation in target tissues. Mutations in this gene are associated with generalized glucocorticoid resistance. Alternative splicing of this gene results in transcript variants encoding either the same or different isoforms. Additional isoforms resulting from the use of alternate in-frame translation initiation sites have also been described, and shown to be functional, displaying diverse cytoplasm-to-nucleus trafficking pat
      • Function:
      • alternative products:At least 4 isoforms, Alpha (shown here), Alpha-B, Beta and Beta-B, are produced by alternative initiation at Met-1 and Met-27. The existence of isoform Alpha and isoform Alpha-B has been proved by mutagenesis. As the sequence environment of the 2 potential ATG initiator codons is the same for the other altrnatively spliced isoforms, alternative initiation of translation could also occur on these transcripts. Additional isoforms seem to exist,disease:Defects in NR3C1 are a cause of glucocorticoid resistance [MIM:138040]; also known as cortisol resistance. It is a hypertensive, hyperandrogenic disorder characterized by increased serum cortisol concentrations. Inheritance is autosomal dominant.,domain:Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal steroid-binding domain.,function:Receptor for glucocorticoids (GC). Has a
      • Subcellular Location:
      • nucleus,nucleoplasm,cytoplasm,mitochondrial matrix,microtubule organizing center,spindle,protein complex,
      • Expression:
      • Fibroblast,Hippocampus,Kidney,Osteosarcoma,Placenta,Uterine
      • Products Images
      • Western Blot analysis of HELA 293T cells using Phospho-GR (S226) Polyclonal Antibody diluted at 1:500
      • Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using GR (Phospho-Ser226) Antibody. The picture on the right is blocked with the phospho peptide.
      • Western blot analysis of lysates from Jurkat cells treated with EGF 200ng/ml 15', using GR (Phospho-Ser226) Antibody. The lane on the right is blocked with the phospho peptide.