PDGFR-β (phospho Tyr771) Polyclonal Antibody

    • Catalog No.:YP0431
    • Applications:WB;IHC
    • Reactivity:Human;Rat
      • Target:
      • PDGFR-β
      • Fields:
      • >>EGFR tyrosine kinase inhibitor resistance;>>MAPK signaling pathway;>>Ras signaling pathway;>>Rap1 signaling pathway;>>Calcium signaling pathway;>>Phospholipase D signaling pathway;>>PI3K-Akt signaling pathway;>>Focal adhesion;>>Gap junction;>>JAK-STAT signaling pathway;>>Regulation of actin cytoskeleton;>>Human papillomavirus infection;>>Pathways in cancer;>>MicroRNAs in cancer;>>Glioma;>>Prostate cancer;>>Melanoma;>>Central carbon metabolism in cancer;>>Choline metabolism in cancer
      • Gene Name:
      • PDGFRB
      • Protein Name:
      • Platelet-derived growth factor receptor beta
      • Human Gene Id:
      • 5159
      • Human Swiss Prot No:
      • P09619
      • Mouse Swiss Prot No:
      • P05622
      • Immunogen:
      • Synthesized phospho-peptide around the phosphorylation site of human PDGFR-β (phospho Tyr771)
      • Specificity:
      • Phospho-PDGFR-β (Y771) Polyclonal Antibody detects endogenous levels of PDGFR-β protein only when phosphorylated at Y771.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Polyclonal, Rabbit,IgG
      • Dilution:
      • WB 1:500-2000;IHC-p 1:50-300
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Other Name:
      • PDGFRB;PDGFR;PDGFR1;Platelet-derived growth factor receptor beta;PDGF-R-beta;PDGFR-beta;Beta platelet-derived growth factor receptor;Beta-type platelet-derived growth factor receptor;CD140 antigen-like family member B;Platelet-deri
      • Observed Band(KD):
      • 135-180kD
      • Background:
      • This gene encodes a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. This gene is flanked on chromosome 5 by the genes for granulocyte-macrophage colony-stimulating factor and macrophage-colony stimulating factor receptor; all three genes may be implicated in the 5-q syndrome. A translocation between chromosomes 5 and 12, that fuses this gene to that of the translocation, ETV6, leukemia gene, results in chronic myeloproliferative disorder with eosinophilia. [provided by RefSeq, Jul 2008],
      • Function:
      • catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,disease:A chromosomal aberration involving PDGFRB is a cause in many instances of chronic myeloproliferative disorder with eosinophilia (MPE) [MIM:131440]. Translocation t(5;12) with ETV6 on chromosome 12 creating an PDGFRB-ETV6 fusion protein.,disease:A chromosomal aberration involving PDGFRB is found in a form of chronic myelomonocytic leukemia (CMML). Translocation t(5;12)(q33;p13) with EVT6/TEL. It is characterized by abnormal clonal myeloid proliferation and by progression to acute myelogenous leukemia (AML).,disease:A chromosomal aberration involving PDGFRB may be a cause of acute myelogenous leukemia. Translocation t(5;14)(q33;q32) with TRIP11. The fusion protein may be involved in clonal evolution of leukemia and eosinophilia.,disease:A chromosomal aberration involving PDGFRB may be a cause
      • Subcellular Location:
      • Cell membrane; Single-pass type I membrane protein. Cytoplasmic vesicle. Lysosome lumen. After ligand binding, the autophosphorylated receptor is ubiquitinated and internalized, leading to its degradation.
      • Expression:
      • Brain,Spleen,
      • Products Images
      • Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
      • Western Blot analysis of mouse lung , rat lung,using primary antibody at 1:1000 dilution. Secondary antibody(catalog#:RS23920) was diluted at 1:10000