PAKγ (phospho Ser141) Polyclonal Antibody

    • Catalog No.:YP0430
    • Applications:WB,IHC-p,IF(paraffin section),ELISA
    • Reactivity:Human,Mouse,Rat
      • Gene Name:
      • PAK2
      • Protein Name:
      • Serine/threonine-protein kinase PAK 2
      • Human Gene Id:
      • 5062
      • Human Swiss Prot No:
      • Q13177
      • Mouse Swiss Prot No:
      • Q8CIN4
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human PAK2 around the phosphorylation site of Ser141. AA range:107-156
      • Specificity:
      • Phospho-PAKγ (S141) Polyclonal Antibody detects endogenous levels of PAKγ protein only when phosphorylated at S141.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Rabbit
      • Dilution:
      • IF(paraffin section) 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -20°C/1 year
      • Other Name:
      • PAK2; Serine/threonine-protein kinase PAK 2; Gamma-PAK; PAK65; S6/H4 kinase; p21-activated kinase 2; PAK-2; p58
      • MolecularWeight(Da):
      • 58004
      • Observed Band(KD):
      • 60
      • Background:
      • p21 (RAC1) activated kinase 2(PAK2) Homo sapiens The p21 activated kinases (PAK) are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. The PAK proteins are a family of serine/threonine kinases that serve as targets for the small GTP binding proteins, CDC42 and RAC1, and have been implicated in a wide range of biological activities. The protein encoded by this gene is activated by proteolytic cleavage during caspase-mediated apoptosis, and may play a role in regulating the apoptotic events in the dying cell. [provided by RefSeq, Jul 2008],
      • Function:
      • catalytic activity:ATP + a protein = ADP + a phosphoprotein.,enzyme regulation:Activated by binding small G proteins. Binding of GTP-bound CDC42 or RAC1 to the autoregulatory region releases monomers from the autoinhibited dimer, enables phosphorylation of Thr-402 and allows the kinase domain to adopt an active structure (By similarity). Following caspase cleavage, autophosphorylted PAK-2p34 is constitutively active.,function:The activated kinase acts on a variety of targets. Phosphorylates ribosomal protein S6, histone H4 and myelin basic protein. Full length PAK 2 stimulates cell survival and cell growth. The process is, at least in part, mediated by phosphorylation and inhibition of pro-apoptotic BAD. Caspase-activated PAK-2p34 is involved in cell death response, probably involving the JNK signaling pathway. Cleaved PAK-2p34 seems to have a higher activity than the CDC42-activated for
      • Subcellular Location:
      • nucleoplasm,cytoplasm,cytosol,plasma membrane,cell-cell adherens junction,perinuclear region of cytoplasm,
      • Expression:
      • Epithelium,Placenta,Platelet,Tongue,
      • Products Images
      • Western blot analysis of lysates from HepG2 cells treated with Adriamycin 0.5uM 24h/Jurkat cells treated with PMA 125ng/ml 30', using PAK2 (Phospho-Ser141) Antibody. The lane on the right is blocked with the phospho peptide.