PAKγ (phospho Ser192) Polyclonal Antibody

    • Catalog No.:YP0429
    • Applications:WB;ELISA
    • Reactivity:Human;Mouse;Rat
      • Target:
      • PAK2
      • Fields:
      • >>MAPK signaling pathway;>>ErbB signaling pathway;>>Ras signaling pathway;>>Axon guidance;>>Focal adhesion;>>T cell receptor signaling pathway;>>Regulation of actin cytoskeleton;>>Pathogenic Escherichia coli infection;>>Human immunodeficiency virus 1 infection;>>Renal cell carcinoma
      • Gene Name:
      • PAK2
      • Protein Name:
      • Serine/threonine-protein kinase PAK 2
      • Human Gene Id:
      • 5062
      • Human Swiss Prot No:
      • Q13177
      • Mouse Swiss Prot No:
      • Q8CIN4
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human PAK2 around the phosphorylation site of Ser192. AA range:158-207
      • Specificity:
      • Phospho-PAKγ (S192) Polyclonal Antibody detects endogenous levels of PAKγ protein only when phosphorylated at S192.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Polyclonal, Rabbit,IgG
      • Dilution:
      • WB 1:500 - 1:2000. ELISA: 1:5000. Not yet tested in other applications.
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Other Name:
      • PAK2;Serine/threonine-protein kinase PAK 2;Gamma-PAK;PAK65;S6/H4 kinase;p21-activated kinase 2;PAK-2;p58
      • Observed Band(KD):
      • 58kD
      • Background:
      • The p21 activated kinases (PAK) are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. The PAK proteins are a family of serine/threonine kinases that serve as targets for the small GTP binding proteins, CDC42 and RAC1, and have been implicated in a wide range of biological activities. The protein encoded by this gene is activated by proteolytic cleavage during caspase-mediated apoptosis, and may play a role in regulating the apoptotic events in the dying cell. [provided by RefSeq, Jul 2008],
      • Function:
      • catalytic activity:ATP + a protein = ADP + a phosphoprotein.,enzyme regulation:Activated by binding small G proteins. Binding of GTP-bound CDC42 or RAC1 to the autoregulatory region releases monomers from the autoinhibited dimer, enables phosphorylation of Thr-402 and allows the kinase domain to adopt an active structure (By similarity). Following caspase cleavage, autophosphorylted PAK-2p34 is constitutively active.,function:The activated kinase acts on a variety of targets. Phosphorylates ribosomal protein S6, histone H4 and myelin basic protein. Full length PAK 2 stimulates cell survival and cell growth. The process is, at least in part, mediated by phosphorylation and inhibition of pro-apoptotic BAD. Caspase-activated PAK-2p34 is involved in cell death response, probably involving the JNK signaling pathway. Cleaved PAK-2p34 seems to have a higher activity than the CDC42-activated for
      • Subcellular Location:
      • [Serine/threonine-protein kinase PAK 2]: Cytoplasm. MYO18A mediates the cellular distribution of the PAK2-ARHGEF7-GIT1 complex to the inner surface of the cell membrane.; [PAK-2p34]: Nucleus. Cytoplasm, perinuclear region. Membrane; Lipid-anchor. Interaction with ARHGAP10 probably changes PAK-2p34 location to cytoplasmic perinuclear region. Myristoylation changes PAK-2p34 location to the membrane.
      • Expression:
      • Ubiquitously expressed. Higher levels seen in skeletal muscle, ovary, thymus and spleen.
      • Products Images
      • Western blot analysis of lysates from HeLa cells, using PAK2 (Phospho-Ser192) Antibody. The lane on the right is blocked with the phospho peptide.