NFκB-p105 (phospho Ser907) Polyclonal Antibody

    • Catalog No.:YP0184
    • Applications:WB,IHC-p,IP,ELISA
    • Reactivity:Human
      • Gene Name:
      • NFKB1
      • Protein Name:
      • Nuclear factor NF-kappa-B p105 subunit
      • Human Gene Id:
      • 4790
      • Human Swiss Prot No:
      • P19838
      • Mouse Swiss Prot No:
      • P25799
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human NF-kappaB p105/p50 around the phosphorylation site of Ser907. AA range:874-923
      • Specificity:
      • Phospho-NFκB-p105 (S907) Polyclonal Antibody detects endogenous levels of NFκB-p105 protein only when phosphorylated at S907.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Rabbit
      • Dilution:
      • Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunoprecipitation: 2-5 ug/mg lysate. ELISA: 1/20000. Not yet tested in other applications.
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -20°C/1 year
      • Other Name:
      • NFKB1; Nuclear factor NF-kappa-B p105 subunit; DNA-binding factor KBF1; EBP-1; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1
      • MolecularWeight(Da):
      • 105356
      • Observed Band(KD):
      • 110
      • Background:
      • nuclear factor kappa B subunit 1(NFKB1) Homo sapiens This gene encodes a 105 kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFKB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFKB has been associated with a number of inflammatory diseases while persistent inhibition of NFKB leads to inappropriate immune cell development or delayed cell growth. Alternative splicing results in multiple transcript variants encoding different isof
      • Function:
      • domain:Glycine-rich region (GRR) appears to be a critical element in the generation of p50.,domain:The C-terminus of p105 might be involved in cytoplasmic retention, inhibition of DNA-binding, and transcription activation.,function:NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Diff
      • Subcellular Location:
      • nucleus,nucleoplasm,cytoplasm,mitochondrion,cytosol,I-kappaB/NF-kappaB complex,
      • Expression:
      • Muscle,Rectum tumor,Uterus,
      • Products Images
      • Western Blot analysis of various cells using Phospho-NFκB-p105 (S907) Polyclonal Antibody diluted at 1:2000
      • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using NF-kappaB p105/p50 (Phospho-Ser907) Antibody.
      • Western blot analysis of lysates from HeLa cells treated with TNF-alpha, using NF-kappaB p105/p50 (Phospho-Ser907) Antibody. The lane on the right is blocked with the phospho peptide.