NFκB-p105 (phospho Ser907) Polyclonal Antibody

    • Catalog No.:YP0184
    • Applications:WB,IHC-p,IP,ELISA
    • Reactivity:Human
    • Description
    • Gene Name:
    • NFKB1
    • Protein Name:
    • Nuclear factor NF-kappa-B p105 subunit
    • Human Gene Id:
    • 4790
    • Human Swiss Prot No:
    • P19838
    • Mouse Swiss Prot No:
    • P25799
    • Immunogen:
    • The antiserum was produced against synthesized peptide derived from human NF-kappaB p105/p50 around the phosphorylation site of Ser907. AA range:874-923
    • Specificity:
    • Phospho-NFκB-p105 (S907) Polyclonal Antibody detects endogenous levels of NFκB-p105 protein only when phosphorylated at S907.
    • Formulation:
    • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
    • Source:
    • Rabbit
    • Dilution:
    • Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunoprecipitation: 2-5 ug/mg lysate. ELISA: 1/20000. Not yet tested in other applications.
    • Purification:
    • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
    • Concentration:
    • 1 mg/ml
    • Storage Stability:
    • -20°C/1 year
    • Other Name:
    • NFKB1; Nuclear factor NF-kappa-B p105 subunit; DNA-binding factor KBF1; EBP-1; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1
    • MolecularWeight(Da):
    • 105356
    • Observed Band(KD):
    • 110
    • Background:
    • nuclear factor kappa B subunit 1(NFKB1) Homo sapiens This gene encodes a 105 kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFKB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFKB has been associated with a number of inflammatory diseases while persistent inhibition of NFKB leads to inappropriate immune cell development or delayed cell growth. Alternative splicing results in multiple transcript variants encoding different isof
    • Function:
    • domain:Glycine-rich region (GRR) appears to be a critical element in the generation of p50.,domain:The C-terminus of p105 might be involved in cytoplasmic retention, inhibition of DNA-binding, and transcription activation.,function:NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Diff
    • Subcellular Location:
    • nucleus,nucleoplasm,cytoplasm,mitochondrion,cytosol,I-kappaB/NF-kappaB complex,
    • Expression:
    • Muscle,Rectum tumor,Uterus,
    • Products Images
    • Western Blot analysis of various cells using Phospho-NFκB-p105 (S907) Polyclonal Antibody diluted at 1:2000
    • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using NF-kappaB p105/p50 (Phospho-Ser907) Antibody.
    • Western blot analysis of lysates from HeLa cells treated with TNF-alpha, using NF-kappaB p105/p50 (Phospho-Ser907) Antibody. The lane on the right is blocked with the phospho peptide.