NFκB-p105 (phospho Ser907) Polyclonal Antibody

    • Catalog No.:YP0184
    • Applications:WB,IHC-p,IP,ELISA
    • Reactivity:Human
    • Description
    • Gene Name:
    • NFKB1
    • Protein Name:
    • Nuclear factor NF-kappa-B p105 subunit
    • Human Gene Id:
    • 4790
    • Human Swiss Prot No:
    • P19838
    • Mouse Swiss Prot No:
    • P25799
    • Immunogen:
    • The antiserum was produced against synthesized peptide derived from human NF-kappaB p105/p50 around the phosphorylation site of Ser907. AA range:874-923
    • Specificity:
    • Phospho-NFκB-p105 (S907) Polyclonal Antibody detects endogenous levels of NFκB-p105 protein only when phosphorylated at S907.
    • Formulation:
    • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
    • Source:
    • Rabbit
    • Dilution:
    • Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunoprecipitation: 2-5 ug/mg lysate. ELISA: 1/20000. Not yet tested in other applications.
    • Purification:
    • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
    • Concentration:
    • 1 mg/ml
    • Storage Stability:
    • -20°C/1 year
    • Other Name:
    • NFKB1; Nuclear factor NF-kappa-B p105 subunit; DNA-binding factor KBF1; EBP-1; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1
    • MolecularWeight(Da):
    • 105356
    • Observed Band(KD):
    • 110
    • Background:
    • NFKB1 encodes a 105 kD protein (nuclear factor kappa B subunit 1) which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFKB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFKB has been associated with a number of inflammatory diseases while persistent inhibition of NFKB leads to inappropriate immune cell development or delayed cell growth. Alternative splicing results in multiple transcript variants encoding different isoforms, at least one of which is proteolytically processed.
    • Function:
    • negative regulation of transcription from RNA polymerase II promoter, regulation of cytokine production, negative regulation of cytokine production, transcription, transcription, DNA-dependent, regulation of transcription, DNA-dependent, regulation of transcription from RNA polymerase II promoter, transcription from RNA polymerase II promoter, proteolysis, apoptosis, anti-apoptosis, defense response, inflammatory response, cell death, macromolecule catabolic process, response to wounding, negative regulation of biosynthetic process, positive regulation of biosynthetic process, regulation of specific transcription from RNA polymerase II promoter, negative regulation of specific transcription from RNA polymerase II promoter, positive regulation of macromolecule biosynthetic process,negative regulation of macromolecule biosynthetic process, positive regulation of macromolecule metabolic pro
    • Products Images
    • Western Blot analysis of various cells using Phospho-NFκB-p105 (S907) Polyclonal Antibody diluted at 1:2000
    • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using NF-kappaB p105/p50 (Phospho-Ser907) Antibody.
    • Western blot analysis of lysates from HeLa cells treated with TNF-alpha, using NF-kappaB p105/p50 (Phospho-Ser907) Antibody. The lane on the right is blocked with the phospho peptide.