IGF-IR (phospho Tyr1165/Y1166) Polyclonal Antibody

    • Catalog No.:YP0139
    • Applications:WB,ELISA
    • Reactivity:Human,Mouse,Rat,Monkey
      • Gene Name:
      • IGF1R
      • Protein Name:
      • Insulin-like growth factor 1 receptor
      • Human Gene Id:
      • 3480
      • Human Swiss Prot No:
      • P08069
      • Mouse Swiss Prot No:
      • Q60751
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human IGF1R around the phosphorylation site of Tyr1165/Tyr1166. AA range:1131-1180
      • Specificity:
      • Phospho-IGF-IR (Y1165/Y1166) Polyclonal Antibody detects endogenous levels of IGF-IR protein only when phosphorylated at Y1165/Y1166.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Rabbit
      • Dilution:
      • Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -20°C/1 year
      • Other Name:
      • IGF1R; Insulin-like growth factor 1 receptor; Insulin-like growth factor I receptor; IGF-I receptor; CD antigen CD221
      • MolecularWeight(Da):
      • 154793
      • Observed Band(KD):
      • 156
      • Background:
      • insulin like growth factor 1 receptor(IGF1R) Homo sapiens This receptor binds insulin-like growth factor with a high affinity. It has tyrosine kinase activity. The insulin-like growth factor I receptor plays a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, May 2014],
      • Function:
      • catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,disease:Defects in IGF1R may be a cause in some cases of resistance to insulin-like growth factor 1 (IGF1 resistance) [MIM:270450]. IGF1 resistance is a gowth deficiency disorder characterized by intrauterine growth retardation and poor postnatal growth accompanied with increased plasma IGF1.,enzyme regulation:Autophosphorylation activates the kinase activity.,function:This receptor binds insulin-like growth factor 1 (IGF1) with a high affinity and IGF2 with a lower affinity. It has a tyrosine-protein kinase activity, which is necessary for the activation of the IGF1-stimulated downstream signaling cascade. When present in a hybrid receptor with INSR, binds IGF1. PubMed:12138094 shows that hybrid receptors composed of IGF1R and INSR isoform Long are activated with a high affinity by IGF1, with low a
      • Subcellular Location:
      • plasma membrane,integral component of plasma membrane,membrane,integral component of membrane,alphav-beta3 integrin-IGF-1-IGF1R complex,intracellular membrane-bounded organelle,receptor complex,
      • Expression:
      • Fetal brain,Lung,Melanocyte,PCR rescued clones,Placenta,
      • Products Images
      • Western Blot analysis of various cells using Phospho-IGF-IR (Y1165/Y1166) Polyclonal Antibody diluted at 1:500
      • Western Blot analysis of COS7 cells using Phospho-IGF-IR (Y1165/Y1166) Polyclonal Antibody diluted at 1:500
      • Western blot analysis of lysates from 293 cells treated with Insulin, using IGF1R (Phospho-Tyr1165/Tyr1166) Antibody. The lane on the right is blocked with the phospho peptide.