IGF-IR (phospho Tyr1165/Y1166) Polyclonal Antibody

    • Catalog No.:YP0139
    • Applications:WB,ELISA
    • Reactivity:Human,Mouse,Rat,Monkey
    • Description
    • Gene Name:
    • IGF1R
    • Protein Name:
    • Insulin-like growth factor 1 receptor
    • Human Gene Id:
    • 3480
    • Human Swiss Prot No:
    • P08069
    • Mouse Swiss Prot No:
    • Q60751
    • Immunogen:
    • The antiserum was produced against synthesized peptide derived from human IGF1R around the phosphorylation site of Tyr1165/Tyr1166. AA range:1131-1180
    • Specificity:
    • Phospho-IGF-IR (Y1165/Y1166) Polyclonal Antibody detects endogenous levels of IGF-IR protein only when phosphorylated at Y1165/Y1166.
    • Formulation:
    • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
    • Source:
    • Rabbit
    • Dilution:
    • Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
    • Purification:
    • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
    • Concentration:
    • 1 mg/ml
    • Storage Stability:
    • -20°C/1 year
    • Other Name:
    • IGF1R; Insulin-like growth factor 1 receptor; Insulin-like growth factor I receptor; IGF-I receptor; CD antigen CD221
    • MolecularWeight(Da):
    • 154793
    • Observed Band(KD):
    • 156
    • Background:
    • Insulin like growth factor 1 receptor binds insulin-like growth factor with a high affinity. It has tyrosine kinase activity. The insulin-like growth factor I receptor plays a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival. Alternatively spliced transcript variants encoding distinct isoforms have been found for IGF1R.
    • Function:
    • cell morphogenesis, cell morphogenesis involved in differentiation, reproductive developmental process, regulation of DNA replication, protein complex assembly, protein amino acid phosphorylation, phosphorus metabolic process,phosphate metabolic process, anti-apoptosis, immune response, establishment or maintenance of cell polarity, cell surface receptor linked signal transduction, enzyme linked receptor protein signaling pathway, transmembrane receptor protein tyrosine kinase signaling pathway, intracellular signaling cascade, protein kinase cascade,axonogenesis, sex determination, positive regulation of cell proliferation, insulin receptor signaling pathway, response to endogenous stimulus, response to hormone stimulus, positive regulation of biosynthetic process, response to organic substance, positive regulation of macromolecule biosynthetic process, positive regulation of macromolec
    • Products Images
    • Western Blot analysis of various cells using Phospho-IGF-IR (Y1165/Y1166) Polyclonal Antibody diluted at 1:500
    • Western Blot analysis of COS7 cells using Phospho-IGF-IR (Y1165/Y1166) Polyclonal Antibody diluted at 1:500
    • Western blot analysis of lysates from 293 cells treated with Insulin, using IGF1R (Phospho-Tyr1165/Tyr1166) Antibody. The lane on the right is blocked with the phospho peptide.